Cassia auriculata Stem Bark Ameliorates the HFD+STZ-Induced Diabetes in both in vitro and in vivo Model

Abstract

Background: The secondary metabolites from natural herbs are exploited to treat various human ailments. The present study aimed to investigate the ameliorative effect of the methanolic extract of Cassia auriculata (C. auriculata) stem-bark in both in vitro and in vivo mice model. Materials and Methods: In vitro standard procedures were followed to determine the phytochemical profile. High-fat-diet+STZ was administered to 6-weeks old mice to induce type-2-diabetes. Results: In vitro study revealed that the highest total polyphenol and flavonoid contents with anti-oxidant and anti-diabetic property was observed in the methanolic extract of C. auriculata stem-bark. Type-2-diabetes rats showed significantly elevated fasting blood glucose levels with decreased serum insulin level. Further, the oxidative stress in the serum and muscle as well as altered activities of liver enzymes of carbohydrate metabolism in diabetic control mice compared to normal control mice was observed. The histomorphology of liver and pancreas was deleteriously altered in diabetic group mice. However, methanolic extract of C. auriculata stem-bark (200 mg/kg; b.wt) and metformin (200 mg/kg; b.wt) treated diabetic mice did not show the above-mentioned alterations suggesting its ameliorative effect in type-2-diabetes. Conclusion: The methanolic extract of C. auriculata is potent enough to be employed as antidiabetic agent.