Quantification of Miconazole Nitrate and Eugenol in Formulated Emulgel by Planar Chromatography along its Stability Studies

Indian Journal of Pharmaceutical Education and Research

  • Aarti Sachin Zanwar1Department of Pharmacy, Sumandeep Vidyapeeth (Deemed to be University), Piparia, Waghodia, Vadodara, Gujarat, INDIA .
  • Dhanya B Sen1Department of Pharmacy, Sumandeep Vidyapeeth (Deemed to be University), Piparia, Waghodia, Vadodara, Gujarat, INDIA .
  • Pruthvi Hiteshkumar Vyas1Department of Pharmacy, Sumandeep Vidyapeeth (Deemed to be University), Piparia, Waghodia, Vadodara, Gujarat, INDIA .
  • Sushil Sureshrao Burle2Department of Pharmaceutical Chemistry, Smt. Kishoritai Bhoyar College of Pharmacy, Kamptee, Nagpur, Maharashtra, INDIA .
  • Dipti Bipinchandra Ruikar3Department of Pharmaceutical Chemistry, P. R. Pote Patil College of Pharmacy, Kathora Naka, Amravati, Maharashtra, INDIA .
  • Ashim Kumar Sen1Department of Pharmacy, Sumandeep Vidyapeeth (Deemed to be University), Piparia, Waghodia, Vadodara, Gujarat, INDIA .

Volume 59 Issue 3s Pages s1134-s1140

DOI: 10.5530/ijper.20255177

Abstract

Introduction: Fungal infections are rapidly increasing and combination therapy using Miconazole Nitrate (MCZ) and Eugenol (EGN), may offer a promising approach for developing new antifungal medications. Objectives: The purpose of the demonstrated work was to develop and validate a stability-indicating HPTLC method for concurrent measurement of MCZ and EGN in formulated emulgel. Materials and Methods: The separation technique of the HPTLC method was aided by pre-coated silica gel 60F254 on aluminium and toluene: ethyl acetate: methyl alcohol (18:1:1 v/v/v) as mobile phase. The quantification of MCZ and EGN in formulated emulgels along with their stability studies was conducted. Results: The optimised conditions were used to develop the spotted drugs and showed a linear response from 100-600 ng/band (r2=0.9995) for MCZ at Rf value of 0.33 and 53-318 ng/band (r2=0.9996) for EGN at Rf value of 0.70. Conclusion: The proposed validated work is suitable for the quantification of the above-cited drugs under various stress conditions and quality control purpose.

Keywords

  • Eugenol
  • Miconazole Nitrate
  • HPTLC
  • Emulgel
  • Topical formulation
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