HPLC Method for Determination of the Chemical Stability of Antimicrobial Peptide α-Defensin 2

Indian Journal of Pharmaceutical Education and Research

  • Vania Maslarska1Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.
  • Meri Amerikova2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.
  • Ivanka Pencheva2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.
  • Stanislav Bozhanov1Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.

Volume 54 Issue 2 Pages 465-470

DOI: 10.5530/ijper.54.2.53

Abstract

Aim: The aim of this research study was to develop a simple, rapid, precise, accurate and economical RP-HPLC method, with a simple mobile phase for the identification and determination of the antimicrobial peptide α-defensin-2. Materials and Methods: Separation was carried out at 25°C, using column Luna 5U (C18, 250x4.6, 5 μm) with mobile phase consisting of acetonitrile: water (40: 60 v/v). The detector was set at 210 nm. The flow rate was 1.2 ml/min and injection volume was 20 μl. Results: The developed method was validated as per ICH guidelines and the kinetic behavior of α-defensin 2 was studied in respect of different pH. Conclusion: The obtained data can serve for creating an in vivo monitoring program and quality control for different studies and preparations.

Keywords

  • Antimicrobial peptides
  • α-defensin 2
  • HPLC
  • Chemical stability
  • Analysis
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