Quantitative Analysis of Pantoprazole Sodium Sesquihydrate in Bulk and Solid Dosage Form via UV-Spectrophotometric Method

Indian Journal of Pharmaceutical Education and Research

  • Asma Rafiq1Faculty of Pharmacy, Jinnah University for Women, Karachi, PAKISTAN.
  • Somia Gul1Faculty of Pharmacy, Jinnah University for Women, Karachi, PAKISTAN.
  • Arzoo Ajaz1Faculty of Pharmacy, Jinnah University for Women, Karachi, PAKISTAN.
  • Sakina Fatima2Institute of Pharmaceutical Sciences, Jinnah Sindh Medical University, Karachi, PAKISTAN.
  • Agha Zeeshan Mirza3Research Laboratories Centre, Faculty of Applied Sciences, Umm Al Qura University, Makkah, SAUDI ARABIA., 4Department of Chemistry, Faculty of Applied Sciences, Umm Al Qura University, Makkah, SAUDI ARABIA.

Volume 54 Issue 2 Pages 448-455

DOI: 10.5530/ijper.54.2.51

Abstract

Pantoprazole sodium sesquihydrate, is a proton pump inhibitor, was analyzed by using UV spectrophotometry. The quantification of pantoprazole sodium sesquihydrate in distilled water was performed in the wavelength range of 290 nm at 20 μgmL-1. The linearity range is 5-35 μgmL-1 by using UV spectrophotometry. The developed method was applied directly and easily to the analysis of the pantoprazole sodium sesquihydrate in bulk and pharmaceutical tablet preparations. The developed method was completely validated according to ICH guidelines. The accuracy of the developed method was calculated in terms of percentage recovery (99.20-101.21%) and %RSD values less than 1% were found in precision. The LOD and LOQ were 0.989 and 1.954 μgmL-1, respectively. Because of simplicity, accuracy and cost-effectiveness, this validated method is helpful for a daily laboratory analysis of pantoprazole sodium sesquihydrate.

Keywords

  • Pantoprazole sodium sesquihydrate
  • Proton pump inhibitor
  • UVSpectrophotometer
  • Method development
  • ICH guidelines
IJOPP

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