LC-MS/MS Determination and Pharmacokinetics Study of Apremilast after Oral Administration in Rabbits

Indian Journal of Pharmaceutical Education and Research

  • Tammisetty Mohan Rao1Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh, INDIA.
  • Challa Balasekhara Reddy2Department of Pharmaceutical Analysis, Vagdevi College of Pharmacy, Gurajala, Guntur, Andhra Pradesh, INDIA.
  • Puttagunta Srinivasa Babu3Department of Pharmaceutics, Vignan Pharmacy College, Vadlamudi, Guntur, Andhra Pradesh, INDIA.

Volume 54 Issue 2 Pages 440-447

DOI: 10.5530/ijper.54.2.50

Abstract

Objectives: A selective and reproducible method has been optimised for evaluation of Apremilast in rabbit biological matrices by UPLC-ESI-MS/MS. Methods: The analytical technique was implemented to quantify the apremilast in rabbit plasma samples with Apremilast-D5 as deuterated internal standard. The chromatographic separation tuned with 10mM Ammonium Acetate Buffer (pH: 4.0): Methanol: Acetonitrile, (20:40:40%, v/v/v) using the CORTECS C18, 2.7 μ.m, 4.6 m.m X 150 m.m analytical column with analysis time four minutes. The flow of mobile phase through column is 0.5 m.L/min. The mass spectrometric ions of Apremilast and Apremilast-D5 obtained were m/z 461.5→257.1 and 466.5→257.1. Results: The curve indicates correlation coefficient (r2) was superior than 0.998 with linear range of 0.03-48.0 n.g/m.L. The developed method was tuned to apply efficaciously for analyzing the pharmacokinetic parameters of Apremilast in rabbit plasma samples. Conclusion: An accurate and reproducible novel method was fabricated for estimation of Apremilast in rabbit biological matrices by UPLC-ESI-MS/MS will be used for regular analysis and appropriate for therapeutic drug monitoring.

Keywords

  • Apremilast
  • Rabbit plasma
  • UPLC-ESI-MS/MS
  • Bio-analysis
  • Pharmacokinetic
  • Therapeutic drug monitoring
IJOPP

Loading…