Pharmacokinetic Differences of Three Aconitum Alkaloids from Aconiti lateralis Radix Praeparata and Compatibility with Pinellia in Rats

Indian Journal of Pharmaceutical Education and Research

  • Wu Jian1Department of Pharmacy, Harbin University of Commerce, Harbin 150076, CHINA., 2Engineering Research Center of Natural Antineoplastic Drugs, Ministry of Education, Harbin 150076, CHINA.
  • Li Wen-lan1Department of Pharmacy, Harbin University of Commerce, Harbin 150076, CHINA., 2Engineering Research Center of Natural Antineoplastic Drugs, Ministry of Education, Harbin 150076, CHINA.
  • Xu Ying1Department of Pharmacy, Harbin University of Commerce, Harbin 150076, CHINA., 2Engineering Research Center of Natural Antineoplastic Drugs, Ministry of Education, Harbin 150076, CHINA.
  • Liu Ying-jie1Department of Pharmacy, Harbin University of Commerce, Harbin 150076, CHINA., 2Engineering Research Center of Natural Antineoplastic Drugs, Ministry of Education, Harbin 150076, CHINA.
  • Liu Ri-jia1Department of Pharmacy, Harbin University of Commerce, Harbin 150076, CHINA., 2Engineering Research Center of Natural Antineoplastic Drugs, Ministry of Education, Harbin 150076, CHINA.
  • Yu Miao1Department of Pharmacy, Harbin University of Commerce, Harbin 150076, CHINA., 2Engineering Research Center of Natural Antineoplastic Drugs, Ministry of Education, Harbin 150076, CHINA.

Volume 53 Issue 2 Pages 236-241

DOI: 10.5530/ijper.53.2.31

Abstract

Aim: This study aims to develop a highly sensitive and specific UPLC-MS/MS method to explore the pharmacokinetic properties of three representative active alkaloids (benzoylhypaconine, benzoylmesaconine, benzoylaconine) after administration of extracts of Aconiti lateralis Radix Praeparata and compatibility with Pinellia, compare the influence of Pinellia on pharmacokinetic characterization of three aconitum alkaloids. Methods: Chromatographic separation were performed on a C18 column under the Multiple reaction monitoring (MRM) in the positive Electrospray ionization (ESI) mode. The pharmacokinetic parameters were evaluated by software DAS 3. 0. Results: The specificity, precision and accuracy,matrix effect and extraction recovery rate and stability all meet the requirements.The validation of the method was achieved in accordance to the FDA guidelines. After oral administration of the extract, similar tendency was found in the mean concentration of the three analytes in the curves, illustrating that three analytes exhibited a similar absorption and metabolic route. Compared the pharmacokinetic characteristics, the AUC0-t, AUC0-∞ and Cmax values of BMA and BHA in Fuzi group were lower than those in Fuzi-Banxia group. Conclusion: This finding indicated the significant statistical decrease (p<0.05) in the absorption of these components. After Banxia in combination with Fuzi, the results showed that faster absorption and higher exposure of BMA and BHA in rat plasma.

Keywords

  • Pharmacokinetic
  • Aconiti lateralis Radix
  • Pinellia
  • Aconitum alkaloids
  • UPLC–MS/MS
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