Development and Validation of Inductively Coupled Plasma Atomic Emission Spectroscopy [ICP-AES] Analytical Method for Estimation of Cisplatin in Biological Samples

Indian Journal of Pharmaceutical Education and Research

  • Shruti Akshay Wagle1Oriental College of Pharmacy, Sector 2, Plot No 3, 4, 5, Sanpada (W), Navi Mumbai, Maharashtra, INDIA.
  • Darshana Jain2C.U.Shah College of Pharmacy, S.N.D.T Women’s University, Santacruz (W), Mumbai, Maharashtra, INDIA.
  • Sudha Rathod1Oriental College of Pharmacy, Sector 2, Plot No 3, 4, 5, Sanpada (W), Navi Mumbai, Maharashtra, INDIA.
  • Rajani Athawale3Principal K.M Kundnani College of Pharmacy, Plot No. 23, Jote Joy Building, Rambhau Salgaonkar Road, Cuffe Parade, Mumbai, Maharashtra, INDIA.
  • Amrita Bajaj4SVKM’s Dr. Bhanuben Nanavati College of Pharmacy, V.M Road, Vile Parle (W), Mumbai, Maharashtra, INDIA.

Volume 51 Issue 4s Pages s783-s789

DOI: 10.5530/ijper.51.4s.113

Abstract

Objective: Cisplatin, a popular anti-neoplastic agent is employed as a first line treatment for variety of cancers. Majority of the analytical methods reported for Cisplatin are either complex or not suitable for routine analysis of the drug. Hence, there is a need for development of suitable analytical technique like ICP-AES for quantitation of Cisplatin in complex matrices and biological samples. Methods: Cisplatin was analyzed in the present study based on its single step conversion to platinum metal with aqua regia. Results: The developed method was validated and was found to be linear in the range of 0.375 to 15 μg/ml with a regression coefficient of 0.992. The percent recoveries of Cisplatin from plasma and tissue samples ranged from 95 to 99%. Discussion: A simple, low cost, sensitive, one step ICP-AES technique was developed and validated for bioanalytical estimation of Cisplatin.

Keywords

  • Cancer
  • Cisplatin
  • ICP-AES
  • Platinum
IJOPP

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