Safflower Polysaccharide Promotes Apoptosis and Inflammatory Response in Gastric Cancer Cells by Up-Regulating miR-6738-3p
Indian Journal of Pharmaceutical Education and Research
Shupei Ma1The Third School of Clinical Medicine (School of Rehabilitation Medicine), Zhejiang Chinese Medical University, Hangzhou, Zhejiang, CHINA., 2Department of Gastroenterology, Zhenjiang Hospital Affiliated to Nanjing University of Chinese Medicine, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang Traditional Chinese Medicine Spleen and Stomach Disease Clinical Medicine Research Center, Zhenjiang, Jiangsu, CHINA.
Dongyu Xie2Department of Gastroenterology, Zhenjiang Hospital Affiliated to Nanjing University of Chinese Medicine, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang Traditional Chinese Medicine Spleen and Stomach Disease Clinical Medicine Research Center, Zhenjiang, Jiangsu, CHINA.
Gang Tao2Department of Gastroenterology, Zhenjiang Hospital Affiliated to Nanjing University of Chinese Medicine, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang Traditional Chinese Medicine Spleen and Stomach Disease Clinical Medicine Research Center, Zhenjiang, Jiangsu, CHINA.
Jing Sun1The Third School of Clinical Medicine (School of Rehabilitation Medicine), Zhejiang Chinese Medical University, Hangzhou, Zhejiang, CHINA., 3Department of Acupuncture and Moxibustion, The Third Affliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, CHINA.
Xiaowen Yu2Department of Gastroenterology, Zhenjiang Hospital Affiliated to Nanjing University of Chinese Medicine, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang Traditional Chinese Medicine Spleen and Stomach Disease Clinical Medicine Research Center, Zhenjiang, Jiangsu, CHINA.
Objectives: To probe the action and mechanism of Safflower Polysaccharide (SPS) on Gastric Cancer (GC) cells. Materials and Methods: qRT-PCR detected miR-6738-3p expression levels. MTT assay and flow cytometry were adopted for the measurement of GC cell survival rate and apoptosis. ELISA analysis examined the levels of inflammatory factors. Results: miR-6738-3p was significantly decreased in GC cell lines, especially in AGS cells, relative to GES-1 cells. SPS treatment dose-dependently reduced the survival rate of AGS cells and induced cell apoptosis and inhibited inflammation. MiR-6738-3p overexpression also induced apoptosis enhancement and inflammation arrest in AGS cells. SPS increased miR-6738-3p content in AGS cells. Moreover, miR-6738-3p deletion reversed the functions mediated by SPS on AGS cell apoptosis promotion and inflammation suppression. Conclusion: Safflower polysaccharide promotes apoptosis and suppresses inflammatory reaction in GC cells by up-regulating miR-6738-3p.
