Optimized Green Synthesis Approaches of Phenytoin Using Divalent Sulfate Salts and Chromatographic Purity Assessment via A Novel RP-HPLC Method
Indian Journal of Pharmaceutical Education and Research
Abstract
Background: Phenytoin is a first-line anti-epileptic drug. Conventionally, It is synthesized by using Benzil and urea in the presence of concentrated HCl. Our aim is to increase the yield as well as the purity of Phenytoin by applying Green approaches and developing an RP-HPLC method for purity assessment. Materials and Methods: 3 different reaction is carried out by using Copper sulfate, Ferrous sulphate and Aluminium sulfate as catalysts along with Benzoin, Sodium nitrate and acetic acid as solvents. Another reaction carried out changing acetic acid with ethanol and the catalyst was Ferrous sulphate. Results and Conclusion: Phenytoin prepared by ferrous sulphate with ethanol gives the highest yield as well as the highest purity compared to other catalysts.
Keywords
- Analytical method development
- Divalent sulfate salts
- Green Synthesis
- Phenytoin
- RP-HPLC