Lentinula edodes Polysaccharides Inhibit Estrogen Biosynthesis Enzymes in Hormone-Dependent Breast Cancer

Indian Journal of Pharmaceutical Education and Research

  • Chandrasekaran Ponnusamy1Department of Biochemistry, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Namakkal, Affiliated to Periyar University, Salem, Tamil Nadu, INDIA.
  • V V Sathibabu Uddandrao2Department of Biotechnology, Karpagam Academy of Higher Education (Deemed to be University), Coimbatore, Tamil Nadu, INDIA., 3Centre for Molecular Therapeutics, Karpagam Academy of Higher Education (Deemed to be University), Tamil Nadu, Coimbatore, INDIA.
  • Vadivukkarasi Sasikumar1Department of Biochemistry, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Namakkal, Affiliated to Periyar University, Salem, Tamil Nadu, INDIA.
  • Puniethaa Prabhu4Department of Biotechnology, K.S. Rangasamy College of Technology, Tiruchengode, Namakkal, Tamil Nadu, INDIA.
  • Saravanan Ganapathy1Department of Biochemistry, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Namakkal, Affiliated to Periyar University, Salem, Tamil Nadu, INDIA.

Volume 60 Issue 3s Pages s1139-s1155

DOI: 10.5530/ijper.20263074

Abstract

Background / Objective: Breast cancer, a leading cause of death among women, is closely associated with estrogen metabolism. This study investigated the potential of partially purified polysaccharides from Lentinula edodes (shiitake mushroom) as natural inhibitors of key enzymes involved in estrogen production aromatase, estrone sulfatase, and 17β hydroxysteroid dehydrogenase which are vital in the progression of hormone-dependent breast cancer. Materials and Methods: Polysaccharide fractions were purified using ion-exchange and size-exclusion chromatography. Enzyme inhibition was assessed through ELISA and colorimetric assays, which revealed concentration-dependent inhibition, with the highest activity observed at 400 µg/mL: aromatase (87.25%, IC₅₀=181.25 µg/mL), estrone sulfatase (86.62%, IC₅₀=187.5 µg/mL), and 17β hydroxysteroid dehydrogenase (86.67%, IC₅₀=208.4 µg/mL). GC-MS analysis identified 33 bioactive compounds, of which 14 were evaluated for pharmacokinetic and drug-likeness properties using SwissADME. Results: Molecular docking showed strong binding interactions between selected compounds (D-fructose and diethyl mercaptal pentaacetate) and the target enzymes, supporting their inhibitory potential. In silico toxicity predictions further confirmed favorable safety profiles. Conclusion: Collectively, these findings highlight L. edodes-derived polysaccharides and associated bioactive molecules as promising natural inhibitors of estrogen biosynthesis, offering new perspectives for therapeutic strategies against hormone-dependent breast cancer.

Keywords

  • Lentinula edodes
  • Breast Cancer
  • Natural products
  • Estrogen biosynthesis Enzymes
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