Influence of Schisandrin A on Serum TNF-α, IL-1β, D-Lactic Acid, Diamine Oxidase and Colonic Tissue Oxidative Stress Markers in a Murine Model of Ulcerative Colitis

Abstract

Introduction: This study was to demonstrate the potential roles and effects of Schisandrin A in the therapy of Ulcerative Colitis (UC). Materials and Methods: 60 mice from the China Experimental Animal Center were purchased and the mice were assigned to Group A (normal control), Group B (UC model), Group C1 (80 mg/kg Schisandrin A), Group C2 (40 mg/kg Schisandrin A), Group C3 (20 mg/kg Schisandrin A) and Group D (sulfasalazine), each comprising 10 individuals. An UC model was established and various parameters including colonic mass, colon length, colon weight index, spleen index and disease activity index were compared across groups. Results: The results revealed marked decreases in colonic mass, colon weight index and spleen index in Group B mice versus Group A. Conversely, colon length, disease activity index, TNF-α, IL-1β, DAO, D-LA and colonic tissue Oxidative Stress (OS) markers showed substantial elevation (p<0.05) in Group B. Comparing Group B with Groups C1, C2, C3 and D indicated varying degrees of improvement in colonic mass, colon length, colon weight index, spleen index, disease activity index, colonic tissue TNF-α, IL-1β levels, as well as serum DAO and D-LA levels and colonic tissue OS markers (p<0.05). Conclusion: In summary, Schisandrin A exhibited the ability to alleviate inflammatory reactions, suppress OS responses and improve symptoms and inflammation severity in UC mice.