Inhibition of miR-224 Repressed Cell Growth, Migration and Invasiveness in Gastric Cancer

Indian Journal of Pharmaceutical Education and Research

  • Changming Shao1Department of Vascular Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, CHINA.
  • Qinshu Shao2Department of Gastrointestinal and Pancreatic Surgery, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou, CHINA.
  • Yingyu Ma3Clinical Research Institute, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou, CHINA.

Volume 60 Issue 3 Pages 1218-1223

DOI: 10.5530/ijper.20263262

Abstract

Aim/Background: MiR-224 has been reported to be associated with many cancers, however, the role of miR-224 in gastric cancer was unclear. Materials and Methods: In the present study, we first used RT-PCR to detect miR-224 level in gastric cancer fresh tissue. Then we performed miR-224 inhibitor transfection in gastric cancer cells, and MTT, cell invasion and migration and colony formation were performed to explore the role of miR-224 in gastric cancer in vitro. Results: The results showed that the relative level of miR-224 in human gastric cancer tissue was significantly higher than that in the adjacent non-tumor gastric mucosa. After transfected by miR-224 inhibitor, the gastric cancer cell proliferation rate was lower compared with the control group, and the migration and invasion are also inhibited by miR-224 inhibitors. The colony number of gastric cancer cells transfected with miR-224 inhibitors was significantly lower than that of the control group. Conclusion: Our results demonstrated that miR-224 is upregulated in gastric cancer. miR-224 inhibitors repressed the proliferation, migration, invasion and colony formation capacity of gastric cancer cells, which indicated that inhibition of miR-224 might be a promising therapeutic option for human gastric cancer.

Keywords

  • miR-224
  • Growth
  • Migration
  • Invasiveness
  • Gastric Cancer
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