Influence of ABCB1 Polymorphisms on Outcomes of Breast Cancer

Indian Journal of Pharmaceutical Education and Research

  • Sambasivam Gopinath1Department of Pharmacology, JIPMER, Puducherry, INDIA., 2Department of Pharmacy Practice, Saveetha College of Pharmacy, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, INDIA.
  • Deepak Gopal Shewade1Department of Pharmacology, JIPMER, Puducherry, INDIA.
  • Biswajit Dubashi3Department of Medical Oncology, JIPMER, Puducherry, INDIA.
  • Ananthakrishnan Ramesh4Department of Radiodiagnosis, JIPMER, Puducherry, INDIA.
  • Saranya Puniyakotti2Department of Pharmacy Practice, Saveetha College of Pharmacy, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, INDIA.
  • Nithya Puniyakotti2Department of Pharmacy Practice, Saveetha College of Pharmacy, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, INDIA.

Volume 60 Issue 1s Pages s245-s251

DOI: 10.5530/ijper.20261224

Abstract

Background: The importance of genotyping ABCB1 polymorphism genes in predicting the outcome of breast cancer patients receiving anthracycline and taxane-based regimens was reported. However, interethnic variations play a significant role in predicting the response to chemotherapy. Materials and Methods: This prospective study was carried out to assess the influence of ABCB1 genotypes on breast cancer response. Genotyping of ABCB1 was carried out by real-time PCR (7300 Applied Biosystems; Life Technologies Corporation, USA) using TaqMan SNP genotyping assays, Response assessments were evaluated as per RECIST criteria version. Results: A total of 170 locally advanced breast cancer patients treated with anthracycline-based neoadjuvant chemotherapy were recruited. The genotype frequencies of ABCB11236 homozygous wild-type variant CC, the heterozygous variant CT, and the mutant homozygous variant TT were 23.0%, 44.0%, and 33.0% respectively. The genotype frequencies of ABCB1 1236 homozygous wild-type variant CC, the heterozygous variant CT, and the mutant homozygous variant TT were 28.0%, 42.0%, and 30.0% respectively. Similarly, the ABCB1 2677 wild-type variant GG (18%), TT (26% AA (2% the heterozygous variant GA (7%), GT (45%), TA (2%), and the mutant homozygous variant were observed. Conclusion: Our study revealed that ABCB1 3435 C>T polymorphism was associated with response as patients with ABCB1 3435 mutant genotypes had better response. Patients having ABCB1 1236 mutant genotypes (CT & TT) had more risk for neutropenia and vomiting, suggesting the significance of ABCB1 genotypes in predicting response and toxicity in anthracycline-based regimens.

Keywords

  • Breast cancer
  • ABCB1
  • Clinical response
  • Neutropenia
  • Multidrug-resistant protein Efflux transporter.
IJOPP

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