A Validated HPTLC Method for Simultaneous Estimation of Berberine and Trigonelline in Polyherbal Mixture A Tool for Quality Control and Standardization

Indian Journal of Pharmaceutical Education and Research

  • Sneha Basangouda Patil1Department of Pharmacognosy, KLE College of Pharmacy Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, INDIA.1Department of Pharmacognosy, KLE College of Pharmacy Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, INDIA.
  • Sunil Satyappa Jalalpure1Department of Pharmacognosy, KLE College of Pharmacy Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, INDIA.1Department of Pharmacognosy, KLE College of Pharmacy Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, INDIA.
  • Priya Prakash Shetti2Dr. Prabhakar Kore Basic Science Research Centre KLE Academy of Higher Education and Research, Belagavi, Karnataka, INDIA.

Volume 60 Issue 1 Pages 431-441

DOI: 10.5530/ijper.20260726

Abstract

Introduction: Berberis aristata and Trigonella foenumgrecum have been used in traditional medicine for various purposes due to their diverse therapeutic benefits such as anti- inflammatory, antidiabetic, antihypertensive and digestive properties. Berberis aristata and Trigonella foenumgrecum consists of bioactive markers Berberine and Trigonelline respectively. Aim: This study aimed to develop a simple, rapid reliable and robust HPTLC method for the simultaneous quantification of biomarkers in polyherbal mixture. Materials and Methods: The separation of these active compounds from the polyherbal mixture was achieved using a mobile phase consisting of toluene: ethyl acetate: formic acid: methanol (6:3:0.3:1v/v/v/v) on pre-coated Silica G 60 F254 plates. Validation of the method encompassed assessments of linearity, accuracy, precision, limits of detection and quantification, robustness and specificity. Results: Berberine and Trigonelline displayed distinct Rf values of 0.88 and 0.29 respectively. The method demonstrated excellent linearity across the concentration range of 2-10 µg/band, with correlation coefficients of 0.9966 and 0.9973 for Berberine and Trigonelline respectively. Conclusion: Percentage recovery was found within the range of 98.84-102.92%. The proposed HPTLC method can be implemented for the concurrent estimation of Berberine and Trigonelline for routine quality control of herbal formulations containing these herbs.

Keywords

  • Berberine
  • Trigonelline
  • Polyherbal mixture
  • HPTLC
  • Analytical method validation
  • Quality control.
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