Development and Validation of UV/visible Spectrophotometric Method for Estimation of Piroxicam from Bulk and Formulation

Indian Journal of Pharmaceutical Education and Research

  • Sandip Mohan Honmane1Department of Pharmaceutics, Annasaheb Dange College of Pharmacy, Ashta, Shivaji University, Kolhapur, Maharashtra, INDIA.
  • Kunal Rajaram Yadav2Department of Pharmaceutical Chemistry, Appasaheb Birnale College of Pharmacy, Sangli, Shivaji University, Kolhapur, Maharashtra, INDIA.
  • Yuvraj Dilip Dange1Department of Pharmaceutics, Annasaheb Dange College of Pharmacy, Ashta, Shivaji University, Kolhapur, Maharashtra, INDIA.
  • Suraj Nijamso Pattekari1Department of Pharmaceutics, Annasaheb Dange College of Pharmacy, Ashta, Shivaji University, Kolhapur, Maharashtra, INDIA.
  • Shradha Shivdayal Tiwari3Department of Pharmaceutics, School of Pharmacy, MIT Vishwaprayag University, Kegaon, Solapur, Maharashtra, INDIA.
  • Mahesh Govind Saralaya4Department of Pharmacology, Annasaheb Dange College of Pharmacy, Ashta, Shivaji University, Kolhapur, Maharashtra, INDIA.

Volume 59 Issue 2s Pages s733-s739

DOI: 10.5530/ijper.20256898

Abstract

Background: Piroxicam (PRX) is a potent anti-inflammatory medicine used both orally and topically to treat arthritis, gout, and other inflammatory disorders. Developing and testing an effective analytical technique is critical for quantitative estimations. Materials and Methods: This study provides a straightforward, exact, repeatable, accurate, and cost-effective UV-visible spectrophotometric method for quantifying PRX in bulk and formulation using Phosphate Buffer Saline (PBS) pH 7.4. In compliance with the International Conference on Harmonization (ICH) guidelines, the developed method was validated for several aspects, including robustness, specificity studies, accuracy, linearity, precision, and range. Results: PRX showed absorbance Maximum (λmax) at 354 nm in PBS pH 7.4. The value of the correlation coefficient (R2) was found to be 0.995 in the range of 2-10 μg/mL. The percent recovery of PRX was found in the range of 98.86-101.44% with less than 2 percentage Relative Standard Deviation (% RSD). The percent recovery from nanosuspension and PRX solution was found to be 99.83% and 100.6% respectively. This indicates that there was no excipient interference. Conclusion: The % RSD for every parameter was less than 2%, which indicated that the developed method was accurate, precise, specific, and suitable for the analysis of commercial samples.

Keywords

  • PRX
  • UV/visible Spectrophotometry
  • Nanosuspension
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