In vitro Evaluation of Curcumin and Quercetin in Combination on Oxidative Stress

Indian Journal of Pharmaceutical Education and Research

  • Archita Katrolia1Department of Pharmaceutics, Amity Institute of Pharmacy, Amity University, Noida, Uttar Pradesh, INDIA.
  • Vedpal2Department of Pharmaceutics, College of Pharmacy, JSS Academy of Technical Education, Noida, Uttar Pradesh, INDIA
  • Vikesh Kumar Shukla3Department of Pharmacognosy, College of Pharmacy, JSS Academy of Technical Education, Noida, Uttar Pradesh, INDIA
  • Rohit Singh4Centre for Drug Regulatory Affairs and Pharmacy Practice, Amity Institute of Pharmacy, Amity University, Noida, Uttar Pradesh, INDIA
  • Sheenu Chandra5Department of Pharmaceutical Chemistry, College of Pharmacy, JSS Academy of Technical Education, Noida, Uttar Pradesh, INDIA
  • Rajeshkumar Raman6Department of Pharmaceutical Biotechnology, JSS College of Pharmacy, Ooty, JSS Academy of Higher Education and Research, Myore, INDIA

Volume 59 Issue 1s Pages s214-s220

DOI: 10.5530/ijper.20254445

Abstract

Background: Flavonoids are the natural product belongs to an important class of plant secondary metabolites. They are polyphenolic compounds and known for decades as a potent natural antioxidant. Curcumin (flavonoid polyphenol) and quercetin (flavonol) both are popular natural antioxidants and contributes a major role as an individual in the treatment of oxidative stress. This research study is more focused in their combination effect against oxidative stress. Materials and Methods: In this present research, we focused on hydro-alcoholic maceration of Curcuma longa rhizomes and Morinda citrifolia fruits to extract the curcumin and quercetin respectively. Also, their role in oxidative stress was studied using in vitro techniques. Results: The percentage (%) yield of hydro-alcoholic extract attained from Curcuma longa rhizomes and Morinda citrifolia fruits were found to be 23.06% and 21.08% respectively. Conclusion: Additionally, through DPPH (2,2-diphenyl-l-picrylhydrazyl) and Lipid peroxidation (LPO) assay, it was observed that curcumin and quercetin in combination gives the highest antioxidant activity when compared with their individual response at same concentration.

Keywords

  • Authentication
  • Hydro-alcoholic maceration
  • Curcumin
  • Quercetin
  • Flavonoids
  • Oxidative stress
IJOPP

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