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IJPER · Lens · Statistically Optimized Nano Emulsion Enhances Bone Regeneration Efficiency of Ipriflavone in Osteoporosis-Induced Zebrafish Model

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Article · Volume 59, Issue 1s (Supplement) · pp. s159-s168

Statistically Optimized Nano Emulsion Enhances Bone Regeneration Efficiency of Ipriflavone in Osteoporosis-Induced Zebrafish Model

Anish John1, Sreehari Malangai Subramanyabhat1, Masmarika Mohan2, Anoop Narayanan Vadakkepushpakath1, Gunimala Chakraborty2, Manohar Mahadev1

  1. 1Nitte (Deemed to be University), NGSM Institute of Pharmaceutical Sciences, Department of Pharmaceutics, Paneer, Deralakatte, Mangalore, Karnataka, INDIA.
  2. 2Nitte (Deemed to be University), Nitte University Centre for Science Education and Research, Department of Molecular Genetics and Cancer, Paneer, Deralakatte, Mangalore, Karnataka, INDIA.

Abstract

Background: The objective of this research was to develop a nano emulsion of ipriflavone (IP nano emulsion), a soy isoflavone to enhance its solubility, bioavailability and bone regeneration potential.Materials and Methods: The oil phase and surfactants for the IP nano emulsion were selected by plotting ternary phase diagrams. Optimisation of surfactant and oil concentrations was achieved using the Box-Behnken Design in Design Expert software. A monodisperse nano emulsion was produced using sonication and the globule size was below 100 nm. The zeta potential readings for the below -15 mV. The optimised formulation was tested for its morphology and examined using TEM imaging.Results: In vitro drug release studies exhibited a release of 80% of ipriflavone in 90 min. The selected formulation demonstrated stability over a three-month period in accelerated conditions of temperature and humidity. The bone regeneration capacity of IP nano emulsion was tested on a dexamethasone-induced osteoporotic zebrafish model. The Caudal vertebrae bone of treated zebrafish showed significant bone regeneration compared to untreated and was confirmed by length measurements.Conclusion: The nano emulsion displayed effective bone regeneration capability in zebrafish models, indicating the usefulness of nano emulsion in the treatment of osteoporosis.

Keywords: Ipriflavone; Nano emulsion; Osteoporosis; Zebrafish model; Bone regeneration

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