Statistically Optimized Nano Emulsion Enhances Bone Regeneration Efficiency of Ipriflavone in Osteoporosis-Induced Zebrafish Model

Indian Journal of Pharmaceutical Education and Research

  • Anish John1Nitte (Deemed to be University), NGSM Institute of Pharmaceutical Sciences, Department of Pharmaceutics, Paneer, Deralakatte, Mangalore, Karnataka, INDIA.
  • Sreehari Malangai Subramanyabhat1Nitte (Deemed to be University), NGSM Institute of Pharmaceutical Sciences, Department of Pharmaceutics, Paneer, Deralakatte, Mangalore, Karnataka, INDIA.
  • Masmarika Mohan2Nitte (Deemed to be University), Nitte University Centre for Science Education and Research, Department of Molecular Genetics and Cancer, Paneer, Deralakatte, Mangalore, Karnataka, INDIA.
  • Anoop Narayanan Vadakkepushpakath1Nitte (Deemed to be University), NGSM Institute of Pharmaceutical Sciences, Department of Pharmaceutics, Paneer, Deralakatte, Mangalore, Karnataka, INDIA.
  • Gunimala Chakraborty2Nitte (Deemed to be University), Nitte University Centre for Science Education and Research, Department of Molecular Genetics and Cancer, Paneer, Deralakatte, Mangalore, Karnataka, INDIA.
  • Manohar Mahadev1Nitte (Deemed to be University), NGSM Institute of Pharmaceutical Sciences, Department of Pharmaceutics, Paneer, Deralakatte, Mangalore, Karnataka, INDIA.

Volume 59 Issue 1s Pages s159-s168

DOI: 10.5530/ijper.20256334

Abstract

Background: The objective of this research was to develop a nano emulsion of ipriflavone (IP nano emulsion), a soy isoflavone to enhance its solubility, bioavailability and bone regeneration potential. Materials and Methods: The oil phase and surfactants for the IP nano emulsion were selected by plotting ternary phase diagrams. Optimisation of surfactant and oil concentrations was achieved using the Box-Behnken Design in Design Expert software. A monodisperse nano emulsion was produced using sonication and the globule size was below 100 nm. The zeta potential readings for the below -15 mV. The optimised formulation was tested for its morphology and examined using TEM imaging. Results: In vitro drug release studies exhibited a release of 80% of ipriflavone in 90 min. The selected formulation demonstrated stability over a three-month period in accelerated conditions of temperature and humidity. The bone regeneration capacity of IP nano emulsion was tested on a dexamethasone-induced osteoporotic zebrafish model. The Caudal vertebrae bone of treated zebrafish showed significant bone regeneration compared to untreated and was confirmed by length measurements. Conclusion: The nano emulsion displayed effective bone regeneration capability in zebrafish models, indicating the usefulness of nano emulsion in the treatment of osteoporosis.

Keywords

  • Ipriflavone
  • Nano emulsion
  • Osteoporosis
  • Zebrafish model
  • Bone regeneration
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