Article · Volume 59, Issue 1 · pp. 94-100

Development and Validation of a High-Performance Liquid Chromatographic Method for Content Assay of Ceftriaxone in Pharmaceutical Dosage

Basavaraj Hiremath¹

¹Department of Chemistry, S. S. Margol College of Arts, Science and Commerce, Shahabad, Kalaburagi, Karnataka, INDIA.

Abstract

Aim: A sensitive and accurate RP-HPLC method was developed and validated for the quantitative measurement of Ceftriaxone sodium (CEF) in pharmaceutical dosage forms and bulk medicine.Materials and Methods: Ceftriaxone sodium was separated on a 245 nm photodiode array detector using a Waters XTerra RP-18 (5 µm 250x4.6 mm internal diameter) column.Results: The method generated an excellent linear response in the concentration range of 0.2-20 µg/mL with remarkable precision of 0.16-0.7% and accuracy in the percent recovery range of 99.88-99.97%.Conclusion: We find the reverse phase HPLC technique to be highly sensitive, accurate, precise and user-friendly and hence recommend it. Therefore, it could be useful in a quality control lab.

Keywords: HPLC; Ceftriaxone; Bulk; Pharmaceutical; Dosage

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