Development and Validation of a High-Performance Liquid Chromatographic Method for Content Assay of Ceftriaxone in Pharmaceutical Dosage

Indian Journal of Pharmaceutical Education and Research

  • Basavaraj HiremathDepartment of Chemistry, S. S. Margol College of Arts, Science and Commerce, Shahabad, Kalaburagi, Karnataka, INDIA.

Volume 59 Issue 1 Pages 94-100

DOI: 10.5530/ijper.20255575

Abstract

Aim: A sensitive and accurate RP-HPLC method was developed and validated for the quantitative measurement of Ceftriaxone sodium (CEF) in pharmaceutical dosage forms and bulk medicine. Materials and Methods: Ceftriaxone sodium was separated on a 245 nm photodiode array detector using a Waters XTerra RP-18 (5 µm 250x4.6 mm internal diameter) column. Results: The method generated an excellent linear response in the concentration range of 0.2-20 µg/mL with remarkable precision of 0.16-0.7% and accuracy in the percent recovery range of 99.88-99.97%. Conclusion: We find the reverse phase HPLC technique to be highly sensitive, accurate, precise and user-friendly and hence recommend it. Therefore, it could be useful in a quality control lab.

Keywords

  • HPLC
  • Ceftriaxone
  • Bulk
  • Pharmaceutical
  • Dosage
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