The Effect of Sophoridine on NSCLC by Down Regulation of mTOR and NOTCH1
Indian Journal of Pharmaceutical Education and Research
Mohammad Raghibul Hasan1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, SAUDI ARABIA.
Ali Hazazi2Department of Pathology and Laboratory Medicine, Security Forces Hospital Program, Riyadh, SAUDI ARABIA., 3College of Medicine, Alfaisal University, Riyadh, SAUDI ARABIA.
Ahad Amer Alsaiari4Department of Clinical Laboratory Science, College of Applied Medical Science, Taif University, Taif, SAUDI ARABIA.
Fawaz M. Almufarriji1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, SAUDI ARABIA.
Amirah Albaqami5Department of Clinical Laboratory Sciences, Turabah University College, Taif University, Taif, SAUDI ARABIA.
Osama Abdulaziz4Department of Clinical Laboratory Science, College of Applied Medical Science, Taif University, Taif, SAUDI ARABIA.
Marwa K Darwish1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, SAUDI ARABIA., 6Department of Chemistry (Biochemistry Branch), Faculty of Science, Suez University, Suez, EGYPT.
Atul Kumar7Department of Biotechnology, Jamia Millia Islamia, Jamia Nagar, New Delhi, INDIA.
Kapil Dev7Department of Biotechnology, Jamia Millia Islamia, Jamia Nagar, New Delhi, INDIA.
Mohammed Ageeli Hakami1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, SAUDI ARABIA.
Introduction: Sophoridine, an alkaloid compound which is isolated from various plant species like Sophora alopecuroides and Sophora flavescens. It identified to have potential pharmacological properties, including anti-inflammatory, anticancer and antiviral activities. The effect of the sophoridine on mTOR and NOTCH1 pathways that are critical in NSCLC is not well understood. Materials and Methods: In order to assess the virtual interaction of mTOR and NOTCH1 with sophoridine, we employed molecular docking. We also performed an in vitro analysis using the NSCLC cell line (A549) using MTT, RT-PCR, western blot and wound healing assays. Results and Discussion: We report strong interaction of sophoridine with mTOR and it exhibited cytotoxic effect on NSCLC cells, causing downregulation of the mTOR and NOTCH1 genes. We used a wound-healing assay to assess its impact on cell migration in NSCLC cells and our findings confirmed that sophoridine significantly (p= 0.0265) inhibits cell migration in A549 cells. Conclusion: Our study indicates sophoridine to be potential anti-cancer drug in NSCLC cell via inhibition of mTOR and NOTCH1 axis.
