Antibacterial Effects of Bioactive Boswellic Acids Loaded Chitosan Nanoparticles against Gram-Positive and Gram-Negative Bacteria

Indian Journal of Pharmaceutical Education and Research

  • Neeta Rathi1Faculty of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana, INDIA.
  • Satyender Kumar2School of Pharmacy, Sharda University, Greater Noida, Uttar Pradesh, INDIA.
  • Seema3Department of Pharmacognosy and Phytochemistry, HIMT College of Pharmacy, Greater Noida, Uttar Pradesh, INDIA.
  • Harish Dureja1Faculty of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana, INDIA., 4Centre for Research Impact & Outcome, Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab, INDIA., 5Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, AUSTRALIA.

Volume 58 Issue 4 Pages 1189-1197

DOI: 10.5530/ijper.58.4.131

Abstract

Background: Boswellic acids are naturally occurring pentacyclic terpenoids that have revealed valuable anti-inflammatory, antiproliferation and anticancerous activities. Instead of these effects boswellic acids also possess antibacterial potential reported in the literature. However, these phytoconstituents associated with low aqueous solubility and bioavailability restrictions. The present study aimed to explore the antibacterial effects of boswellic acids by means of nano formulations. Materials and Methods: Chitosan was utilized as a natural biocompatible material for nanoparticles preparation, employing the ionic gelation technique as an effective approach. Well diffusion method was used to test antibacterial activity against four pathogenic bacteria (Gram-positive bacteria: Staphylococcus aureus, Bacillus subtilis; Gram-negative Salmonella typhi, and. Escherichia coli. Micro broth dilution technique was used to determine the minimum inhibitory concentration. Results: Boswellic acids-loaded nanoparticles displayed spherical particles with particle size 104.6 nm and 0.081 PDI value, respectively with smooth-surfaced spherical particles. Boswellic acids chitosan nanoparticles have a greater zone of inhibition against Salmonella typhi than Boswellia serrata extract with MIC values of 3.91 and 7.81 μg/ mL, respectively. Conclusion: Poorly soluble boswellic acids were successfully encapsulated in chitosan nanoparticles and exhibited improved antibacterial activity compared to Boswellia serrata extract.

Keywords

  • Boswellic acids
  • Chitosan nanoparticles
  • Antibacterial activity
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