Simultaneous Determination of Amitriptyline Hydrochloride and Propranolol Hydrochloride in Commercial Formulation by Multitudinal UV and Multivariate FT-IR Spectroscopic Methods

Indian Journal of Pharmaceutical Education and Research

  • Sreenivasa Charan Archakam1Department of Pharmaceutical Analysis, Sri Padmavathi School of Pharmacy, Mohan Gardens, Tiruchanoor, Andhra Pradesh, INDIA.
  • Keerthisikha Palur1Department of Pharmaceutical Analysis, Sri Padmavathi School of Pharmacy, Mohan Gardens, Tiruchanoor, Andhra Pradesh, INDIA.
  • Rajitha Galla2Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, INDIA.
  • Sai Sucharitha Padiri1Department of Pharmaceutical Analysis, Sri Padmavathi School of Pharmacy, Mohan Gardens, Tiruchanoor, Andhra Pradesh, INDIA.
  • Praveen Kumar Tulasi1Department of Pharmaceutical Analysis, Sri Padmavathi School of Pharmacy, Mohan Gardens, Tiruchanoor, Andhra Pradesh, INDIA.
  • Ranganayakulu Diviti3Department of Pharmacology, Sri Padmavathi School of Pharmacy, Mohan Gardens, Tiruchanoor, Andhra Pradesh, INDIA.

Volume 58 Issue 2s Pages s552-s557

DOI: 10.5530/ijper.58.2s.57

Abstract

Aim: In the current study, a multivariate FTIR method and two different UV spectrophotometric methods were employed for the simultaneous determination of Amitriptyline Hydrochloride (ATH) and Propranolol Hydrochloride (PPH) in their combined formulation. Materials and Methods: The multivariate FTIR method based on the use of Classical Least Squares (CLS) was developed and executed in the lab solutions software for the simultaneous determination of ATH and PPH. CLS model was performed in the wavenumber range of 2550.04-3600.12 cm-1 and 969.64-1757 cm-1. In addition, two UV spectroscopic methods, namely absorbance correction method and crammer’s matrix method were developed and validated in the concentration ranges of 2-10μg/mL and 5-55μg/mL for ATH and PPH respectively. Results: The statistical parameters obtained from the applied multivariate FTIR-CLS model revealed the model accuracy and the assay results of ATH and PPH were found to be 92.32 (%w/w) and 97.35 (%w/w) respectively. The developed UV spectroscopic methods were validated as per ICH guidelines and all the validation parameters were found to be within the acceptance criteria. Conclusion: The methods employed in the current study were found to simple, economical, accurate and do not require any prior separation.

Keywords

  • Classical least squares
  • Absorbance correction method
  • Crammer’s matrix method
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