Interaction Studies of Moxifloxacin with Anti-diabetic Drugs and Application of RP-HPLC in Analysis

Indian Journal of Pharmaceutical Education and Research

  • Sania Bashir1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jinnah University for Women, Karachi, PAKISTAN.
  • Fatima Qamar1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jinnah University for Women, Karachi, PAKISTAN.
  • Safila Naveed1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jinnah University for Women, Karachi, PAKISTAN.
  • Syeda Zainab2Department of Pharmaceutics, Jinnah University for Women, Karachi, PAKISTAN.
  • Halima Sadia1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jinnah University for Women, Karachi, PAKISTAN.
  • Aisha Sana1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jinnah University for Women, Karachi, PAKISTAN.
  • Wajeeha Muzafar3Department of Chemistry, H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, PAKISTAN.

Volume 57 Issue 4 Pages 1226-1231

DOI: 10.5530/ijper.57.4.146

Abstract

Aim: Simple RP-HPLC method was developed and validated for the simultaneous estimation of Glimepiride, Glibenclamide and Moxifloxacin in pharmaceutical preparation. Materials and Methods: Mediterranean C18 column with dimensions of 150mm×4.6mm was used with HPLC Shimadzu LC-20 AT model, Version 1:62 is used for processing of chromatograms at room temperature (25± 2°C). 1 mL per minute was the flow rate and detector were set at 254nm with mobile Phase consisting of 85:10:5 v/v methanol: water: acetonitrile. Results: The developed method proved to be accurate, simple, effective precise and reproducible. One way ANOVA was also applied for further confirmation of the results. Conclusion: The method is applicable for the routine analysis of drugs in API and in formulation and for studying in vitro interaction studies.

Keywords

  • Moxifloxacin
  • Glimepiride
  • Glibenclamide
  • in vitro interaction studies
IJOPP

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