Binding of Novobiocin Paves the Way for Inhibition of DEAH-box Helicase 8

Indian Journal of Pharmaceutical Education and Research

  • Mohammad Rehan Ajmal1 Department of Biochemistry, Physical Biochemistry Research Laboratory, Faculty of Science, University of Tabuk, Tabuk, SAUDI ARABIA.
  • Fahad Mohammad Almutairi1 Department of Biochemistry, Physical Biochemistry Research Laboratory, Faculty of Science, University of Tabuk, Tabuk, SAUDI ARABIA.
  • Arif Hussain2 School of Life Sciences, Manipal Academy of Higher Education-Dubai Campus, Dubai, UNITED ARAB EMIRATES.
  • Mohammad Fahad Ullah3 Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, SAUDI ARABIA.
  • M Ayaz Ahmad4 Department of Physics, Faculty of Science, University of Tabuk, SAUDI ARABIA., 5 Department of Mathematics, Physics and Statistics, University of Guyana, South America, 521 Good Hope Coast Demerara, Georgetown, GUYANA.
  • Ali Saber Abdelhameed6 Department of Pharmaceutical Chemistry, College of Pharmacy, King Saudi University, Riyadh, SAUDI ARABIA.
  • Hussain Arif7 Department of Biochemistry, Mohammad Ali Jauhar University, Rampur, Uttar Pradesh, INDIA.

Volume 57 Issue 2s Pages s459-s464

DOI: 10.5530/ijper.57.2s.54

Abstract

Introduction: DEAH-box helicase 8 (DHX8) is a crucial DEAH-box RNA helicase involved in splicing and required for the release of mature mRNA from the splice. Here, we report the interaction study of human DHX8 and four test compounds namely etoposide, netropsin, nogalamycin, and novobiocin. Materials and Methods: Molecular docking and fluorescence emission spectra techniques were employed to determine the binding and inhibitory effect of test compounds. Results: Our docking and fluorescence emission spectra results showed that DHX8 has a good binding preference for novobiocin among these four test compounds. Moreover, fluorescence emission spectra of DHX8 with novobiocin also revealed the 2.5 µM concentration is an effective novobiocin concentration to inhibit the activity of DHX8. Conclusion: These findings provide an in-depth understanding of the inhibition of DHX8 and contribute insights towards the development of novobiocin as a therapeutic molecule against the DHX8 in targeted diseases.

Keywords

  • DEAH-box helicase
  • DHX8
  • Novobiocin
  • Splicing
  • Therapeutics
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