Rutin Inhibits Hepatic and Pancreatic Cancer Cell Proliferation by Inhibiting CYP3A4 and GST

Indian Journal of Pharmaceutical Education and Research

  • Bandar A Alyami1 Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran, SAUDI ARABIA.
  • Mohammad Zaki1 Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran, SAUDI ARABIA.
  • Mahmoud Youns2 Department of Biochemistry and Molecular biology, Faculty of Pharmacy, Helwan University, Cairo, EGYPT.
  • Bassim Amin3 Department of Pharmacology, Medical Research and Clinical Trial Institute, National Research Centre, Giza, EGYPT.
  • Randa Abdou4 Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah, SAUDI ARABIA.
  • Mohamed Dawoud4 Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah, SAUDI ARABIA.
  • Gouda H Attia1 Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran, SAUDI ARABIA.

Volume 57 Issue 2s Pages s411-s418

DOI: 10.5530/ijper.57.2s.48

Abstract

Background: Digestive cancer is among the major causes of mortality and morbidity worldwide. Rutin, a bioactive secondary metabolite belonging to flavonoids and distributed in many fruits and vegetables has shown anti-proliferative, anti-cancer, and neuroprotective activities. In this study, the antiproliferative, antioxidant and apoptotic activities of rutin on hepatic and pancreatic cancer cell lines were investigated. Materials and Methods: The effect on cellular viability was monitored by SRB assay. Increasing activity of caspases (3/7) was used as an indicator of apoptosis. Additionally, the anti-inflammatory and antioxidant activities of rutin were evaluated after measuring amount of prostaglandin E2 (PGE2) produced and through DPPH free radical scavenging assays, respectively. Moreover, the inhibitory effect on both CYP3A4 and GST enzymes has also been evaluated. Results: According to the data presented here, rutin has anti-proliferative effect and raises the number of caspases 3/7 in investigated cell lines. Conclusion: HepG-2 cells showed the highest cellular growth inhibition, followed by BxPC-3, Huh-7, MiaPaCa-2, Suit-2, and the normal cell line HPDE. Rutin also inhibited the cyctochrome P450 enzyme (CYP450 3A4) and glutatihione-S-transferase activity, with dose-dependent inhibition. Furthermore, suppression of PGE2 synthesis in BxPC-3 cells supported rutin's anti-inflammatory action (high COX-2 expression).

Keywords

  • Rutin
  • Pancreatic and Liver cancer
  • Apoptosis and Anti-inflammatory
  • Antioxidant
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