Toxicological Study of n-hexane and Chloroform Extracts of Lantana camara L. in Experimental Animals

Indian Journal of Pharmaceutical Education and Research

  • Sribatsa Lanchhana Dash1 Department of Pharmaceutical Sciences, Utkal University, Vani Vihar, Bhubaneswar, Odisha, INDIA.
  • Ranjit Mohapatra1 Department of Pharmaceutical Sciences, Utkal University, Vani Vihar, Bhubaneswar, Odisha, INDIA.
  • Suman Kumar Mekap1 Department of Pharmaceutical Sciences, Utkal University, Vani Vihar, Bhubaneswar, Odisha, INDIA., 2 Department of Pharmacy, Centurion University of Technology and Management, Bhubaneswar, Odisha, INDIA.
  • Smruti Ranjan Masanta3 School of Pharmaceutical Sciences, Siksha 'O' Anusandhan University, Bhubaneswar, Odisha, INDIA.
  • Arpit Katiyar4 Sai Meer College of Pharmacy, Chhibramau, Kannauj, Uttar Pradesh, INDIA.
  • Nitin Sharma5 Department of Pharmaceutics, ISF College of Pharmacy, Ghal Kalan, Moga, Punjab, INDIA.
  • Ritu Karwasra6 Central Council for Research in Unani Medicine, Ministry of AYUSH, Janakpuri, New Delhi, INDIA.
  • Sagar Kumar Mishra1 Department of Pharmaceutical Sciences, Utkal University, Vani Vihar, Bhubaneswar, Odisha, INDIA.

Volume 57 Issue 2s Pages s370-s380

DOI: 10.5530/ijper.57.2s.43

Abstract

Aim: The aim of the current study is to investigate the acute, sub-acute and sub-chronic toxicity study of extracts (n-hexane and chloroform) of L. camara in Wistar rats. Materials and Methods: HPTLC and GC-MS analysis were carried out to analyze different constituents present in L. camara extract. Acute toxicity study was conducted as per OECD 420 guidelines, while sub-acute and sub-chronic studies were conducted according to OECD 407 and 408 guidelines respectively. Toxicopathological effects of both extracts were tested with the help of hemotoxylin and eosin staining of vital organs (liver, kidney, brain, testes, and ovaries) in experimental animals. Antioxidant and behavioral tests were also conducted on both the extracts, so as to evaluate the possible effects of toxicity. Results: The toxicity investigation of chloroform and n-hexane extracts exhibits no toxicity in terms of hematological (p<0.01) and biochemical parameters (p<0.05). Histopathological sections also confirmed the non-toxic effects on the liver, brain, kidney and on reproductive organs. Conclusion: The n-hexane and chloroform extracts of L. camara can be safely used for therapeutic purposes.

Keywords

  • Lantana camara
  • GC-MS
  • HPTLC
  • Toxicity
  • Histopathology
  • Antioxidant
  • PI uptake
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