Identification of Antioxidant and Antidiabetic Compounds in Hedychium spicatum using TLC Bioautography Coupled with Mass Spectrometry and their in silico Molecular Docking Studies

Indian Journal of Pharmaceutical Education and Research

  • Harjeet Singh1CARI, CCRAS (Ministry of AYUSH) Jhansi Uttar Pradesh, INDIA.
  • Nardev Singh2Graphic Era Hill University, Dehradun, Uttarakhand, INDIA.

Volume 56 Issue 3 Pages 830-837

DOI: 10.5530/ijper.56.3.135

Abstract

Background: Traditional system of medicine has been practised since a long time to cure various diseases. More than 60-80 percent people across the world use herbal medicine for their health care. In the present study, TLC-MS bioautography combined with DPPH, α-amylase and α-glucosidase bioassay were used to compare antidiabetic and antioxidant activities in hydro alcoholic extracts of H. spicatum and also explore in silico molecular docking studies. Materials and Methods: In this paper, hydro-alcoholic extract of H. spicatum was prepared, run TLC and performed TLC- MS bioautography to identify antidiabetic and antioxidant bioactive compounds. Results: HPTLC analysis showed there are 10 and 5 metabolites present at 254 nm and 366 nm respectively in H. spicatum. Further, TLC-MS bioautography studies isolate total two lead compounds such as quercetin and beta-sitosterol. Quercetin shows DPPH, α-amylase and α-glucosidase activity whereas beta sitosterol shows α amylase activity only. Conclusion: In conclusion, TLC-MS bioautography proved to be a good technique to evaluate the antidiabetic and antioxidant potential of plant extracts and will be useful for discovering new therapeutic regimens.

Keywords

  • Hedychium spicatum
  • TLC-bioautography
  • Antioxidant
  • Antidiabetic
  • in silico
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