Formulation, Characterization, and Evaluation of Doxorubicin-loaded Cubosome as a Cytotoxic Potentiator against HCT-116 Colorectal Cancer Cells

Indian Journal of Pharmaceutical Education and Research

  • Yosif Almoshari1Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, SAUDI ARABIA.
  • Mohammad Intakhab Alam1Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, SAUDI ARABIA.
  • Mohammed Ali Bakkari1Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, SAUDI ARABIA.
  • Ahmad Salawi1Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, SAUDI ARABIA.
  • Meshal Alshamrani1Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, SAUDI ARABIA.
  • Fahad Y Sabei1Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, SAUDI ARABIA.
  • Awaji Y Safhi1Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, SAUDI ARABIA.
  • Jawaher Abdullah Alamoudi2Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh, SAUDI ARABIA.
  • Ibrahim M Ibrahim3Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah, SAUDI ARABIA.

Volume 56 Issue 3 Pages 723-731

DOI: 10.5530/ijper.56.3.121

Abstract

Background: Colorectal cancer (CRC) has grown to be the world’s fourth-biggest cause of cancer-related mortality. It is critical to identify more effective treatment options for it. Objectives: Doxorubicin a potent antineoplastic agent is widely used but has severe adverse effects. Therefore, our objective is to use cubosome as an efficient drug delivery system to reduce the off-target effects and increase the cytotoxic effects in CRC cells. Materials and Methods: Pluronic F127-Based Cubosomes were prepared by low energy stirring method and loaded with doxorubicin. Physicochemical characteristics were studied using X-ray diffraction, dynamic light scattering, microscopic techniques, FTIR UV spectrophotometer, and entrapment efficiency. In vitro activity of doxorubicin-loaded cubosomes was studied on HCT-116 cells. Results: The prepared cubosomes have the required nano-size and the FTIR results showed the presence of both doxorubicin and Pluronic F-127. The entrapment efficiency of doxorubicin was high too. DOX-CBs have a better cytotoxic effect and induced ROS and reduced NO levels in HCT-116 cells. Conclusion: The obtained results show that doxorubicin-loaded cubosomes can be used to increase the cytotoxic effect in CRC cells and can be used with other chemotherapeutic agents.

Keywords

  • Doxorubicin
  • HCT-116 cells
  • Pluronic F127
  • in vitro activity
  • Cubosomes
  • Entrapment efficiency
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