Biogenic Synthesis of Silver Nanoparticles (AgNPs) using Solanum indicum Linn.

Indian Journal of Pharmaceutical Education and Research

  • Sadeq Khalid Alhag1Biology Department, College of Science and Arts, King Khalid University, Muhayl Asser, SAUDI ARABIA., 2Biology Department, College of Science, Ibb University, YEMEN.
  • Hamed Ali Ghramah3Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, SAUDI ARABIA., 4Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, SAUDI ARABIA.
  • Lamya Ahmed Al-keridis5Department of Biology, Faculty of Science, Princess Nourah bint Abdulrahman University, Riyadh, SAUDI ARABIA.
  • Fahd Abdu Al-Mekhlafi6Bioproducts Research Chair, Department of Zoology, Faculty of Science, King Saud University, SAUDI ARABIA., 7Department of Agricultural Production, College of Agriculture and Veterinary Medicine, Thamar University, YEMEN.
  • Essam Hassan Ibrahim3Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, SAUDI ARABIA., 8Blood Products Quality Control and Research Department, National Organization for Research and Control of Biologicals, Cairo, EGYPT., 9Unit of Bee Research and Honey Production, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, SAUDI ARABIA.
  • Khalid Ali Khan3Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, SAUDI ARABIA.

Volume 55 Issue 1s Pages S202-S208

DOI: 10.5530/ijper.55.1s.51

Abstract

Background: Synthesis of silver nanoparticles (AgNPs) using green plants is important for biocompatibility, reduced hazards, green policy and eco-friendliness. Materials and Methods: In this study, a leaf extract of Solanum indicum and associated AgNPs were used to examine larvicidal properties and other biological activities. AgNP forms were characterized with scanning electron- microscopy (SEM), Fourier Transform Infrared Radiation spectroscopy (FTIR) and UV-vis spectra. Larvicidal activity was assessed with Culex pipiens larvae using the World Health Organization method. Antibacterial potential of S. indicum leaf extract and AgNPs were evaluated with a well diffusion assay. In vitro impacts of S. indicum extract and AgNPs were studied using spleen cell propagation. Results: S. indicum ethanol extract and associated AgNPs show larvicidal activity against 4th instar of Cx. pipiens. Synthesized AgNPs were more toxic, with lower lethal concentration values, (LC50 = 47.181 ppm; LC90 = 243.776ppm) compared to plant extract (LC50 = 131.448 ppm; LC90 = 4397.528 ppm) after a twenty-four hour of exposure. Synthesized AgNPs also show significant impact Staphylococcus aureus, Proteus mirabilis, Escherichia coli and Shigella flexneri. Cytotoxic effects were demonstrated for S. indicum extract and AgNPs on normal spleen cells. The extract and associated AgNPs were also safe for red blood cells (RBCs) and failed to cause substantial elevation in serum liver enzymes. Conclusion: Leaf extracts of S. indicum and associated AgNPs may be efficient and eco-friendly for control of Cx. pipiens. Their antibacterial activity may be useful if care is taken to prevent cytotoxic effects.

Keywords

  • Cytotoxic effect
  • Culex pipiens
  • Silver Nanoparticles
  • Antibacterial
  • Larvicidal
  • Solanum indicum
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