Doxorubicin Hydrochloride Loaded Polyanhydride Nanoformulations and Cytotoxicity
Indian Journal of Pharmaceutical Education and Research
Nagaraja Sreeharsha1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA., 2Department of Pharmaceutics, Vidya Siri College of Pharmacy, Off Sarjapura Road, Bangalore, Karnataka, INDIA.
Jagadeesh G Hiremath3Department of Pharmaceutics, PA College of Pharmacy, Mangalore, Karnataka, India.
Prashanth Kumar R4Department of Pharmaceutics, East West College of Pharmacy, Bangalore, Karnataka, INDIA.
Girish Meravanige5Department of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa, SAUDI ARABIA.
Saleemulla Khan6Department of Pharmacognosy, PA College of Pharmacy, Mangalore, Karnataka, INDIA.
Ranjith Kumar Karnati7Department of Chemistry, College of Science, King Faisal University, Hofuf, SAUDI ARABIA.
Mahesh Attimarad1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA.
Bandar Al-Dhubiab1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA.
Anroop Balachandran Nair1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA.
Katharigatta Narayanaswamy Venugopala1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA., 8Department of Biotechnology and Food Technology, Durban University of Technology, Durban, SOUTH AFRICA.
This study aimed to evaluate DOX∙HCl loaded P(DLLA-CO:60:40) nanoformulations with a copolymer of F127 as a potential drug delivery system for cancer. DOX ∙ HCl was encapsulated in nanoformulations of Poly DL-lactic acid co castor oil 60:40, P(DLLACO: 60:40) polymer with the copolymer/stabilizer Pluronic® F127. The mean diameter of the cylindrical rod shape of the nanoformulation particles was 248±2.4-260±3.2 nm with an acceptable poly dispersibility of 0.29- 0.33 and a smooth surface as visualized by DSC, SEM and XRD displayed that DOX∙HCl was present as an disordered crystalline or amorphous state in the nanoformulations. The nanoformulations showed a steady pattern of drug discharge for 24 hrs. F-3 and F-4 formulations had IC50 values of 3.2±0.03 and 1.98±0.08 mcg/ml while the free drug inhibition concentration of IC50 was 2.2 mcg/ml. The drug-loaded nanoformulations showed significant cytotoxic effects on MCF-7 breast cancer cell lines.
