In-vitro Cytotoxicity and in silico Molecular Docking of Alkaloids from Tiliacora acuminata

Indian Journal of Pharmaceutical Education and Research

  • Jeswiny Rodrigues1Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.
  • Kirankumar Hullatti1Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.
  • Sunil Jalalpure1Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.
  • Pukar Khanal2Department of Pharmacology and Toxicology, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.

Volume 54 Issue 2s Pages s295-s300

DOI: 10.5530/ijper.54.2s.86

Abstract

Background: The present study aimed to identify the cytotoxicity of Tiliacora acuminata extract/fraction(s) plant via bioactivity guided approach and predict the binding affinity with Topoisomerase II. Materials and Methods: Extract and fractions were screened using brine shrimp lethality bioassay and the potent fraction was further evaluated for its in vitro cytotoxicity using five different cell lines i.e. HT-29, HepG2, MCF-7 and A-549. The binding affinity of individual phytoconstituent from the potent fraction with Topoisomerase II was further predicted using autodock4. Results: The fraction containing alkaloids showed the highest cytotoxicity against the HT-29 cell line. Molecular docking study identified coclaurine as a potent alkaloid phytoconstituent to bind with topoisomerase II. Conclusion: The study revealed the potential cytotoxic alkaloids from T. acuminata which may find use in the development of cytotoxic or chemopreventive agents.

Keywords

  • Alkaloids
  • Brine shrimp
  • Docking
  • Menispermaceae
  • MTT
  • Topoisomerase II
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