Prolonged in vivo Stinging Nettle Treatment Impacts on Functional Capacity of Leukocytes in Immunologically Mature Chickens
Indian Journal of Pharmaceutical Education and Research
Șandru Carmen Dana1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Niculae Mihaela1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Pall Emoke1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Brudaşcă Florinel1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Vasiu Aurel1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Silvana Popescu1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Cerbu Constantin1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Giupana Radu1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Vasiu Constantin1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Valentin Tudor1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Spînu Marina1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Objective: The experiment aimed at establishing the effects of an alcoholic Urtica dioica
extract on the in vitro blastogenic response in antigen stimulated, immunologically
mature chickens. Materials and Methods: Three equal groups (n=17) of 47 days old,
Rock x Cornish chickens were subjected to oral administration of: a) 0.5 ml/chicken/
day of an alcoholic stinging nettle extract (I), b) 0.5 ml/chicken/day alcohol (II, solvent
control), and c) 0.5 ml chicken/day water (III-environment control) for seven days. All
birds were injected with 0.5 ml/bird of a 5% SRBC suspension (days 0 and 7). Leukocyte
numbers (Burker Turk method) and their blastogenic capacity (blast transformation test)
were monitored on days 0, 7 and 14. The specific response was evaluated against a
SRBC lysate. Student’s t-test was used to evaluate the statistical significance of the
differences. Results and Discussion: The leukocyte numbers increased to from 18,083.33
±4,879.81/mm3 to 42,833.33±7,547.99/mm3 by day 14. In vitro responses to
C. officinalis and E. angustifolia decreased while SRBC response increased in the nettle
treated group. Conclusion: The results did not validate the implemented protocol for the
alcoholic stinging nettle treatment. Other administration routes, schemes or dosages
should be tested to improve the functional capacity of the leukocytes.
