Seasonal Changes of Phagocytic Activity Enhanced by an Acoholic Mint Extract in Cultured Rainbow Trout
Indian Journal of Pharmaceutical Education and Research
Spînu Marina1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Pall Emoke1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Niculae Mihaela1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Vasiu Aurel1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Brudaşcă Gheorghe Florinel1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Cerbu Constantin Gheorghe1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Negrutiu Vlad1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Vasiu Constantin1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Silvana Popescu1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Sandru Carmen Dana1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Objective: We hypothesized that leukocyte numbers and their in vitro phagocytic activity
in farmed rainbow trout are subject to negative influence of extreme temperatures for
fish and that mint extract could alleviate these effects. Materials and Methods: Blood
samples from farmed rainbow trout collected during winter and summer were subjected
to leukocyte subpopulation counts (%) and carbon particle inclusion test to estimate
the in vitro phagocytic activity after 15 and 30 min of incubation at 4 and 22°C, with
or without alcoholic mint extract (ln). Results and Discussion: There was a significant
increase in lymphocytes (p<0.05) and monocytes (p<0.01) in summer versus winter,
while the heterophils were dominant during winter (p<0.01). In vitro spontaneous
phagocytosis increased with the seasonal increase in temperature (0.425±0.094, winter
and 0.835±0.102, summer, p<0.05) and during the first reading period (0 to 15 min,
p<0.01). The mint extract increased phagocytosis for the overall reading period from 2x
(winter, 0.281±0.221) up to 5x (summer, 1.032±0.221) (p<0.01). Conclusion: The
positive activity of the alcoholic mint extract during phagocytosis, more intense during
summer than during winter, proved to be a temperature driven process, plant active
principles playing a dual stimulating role for both monocytes and heterophiles.
