Comparison of Mean Centering of Ratio Spectra Based Spectrophotometric Approach and HPLC Method for Quantitative Determination of Pirenoxine in the Presence of Methylparaben and Propylparaben
Indian Journal of Pharmaceutical Education and Research
Hendri Wasito1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University; 447 Sri-Ayuthaya Road, Phayathai, Ratchathewi, Bangkok, THAILAND., 2Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University; Dr. Soeparno Road, Karangwangkal, Purwokerto, INDONESIA.
Sawanya Buranaphalin1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University; 447 Sri-Ayuthaya Road, Phayathai, Ratchathewi, Bangkok, THAILAND.
Leena Suntornsuk1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University; 447 Sri-Ayuthaya Road, Phayathai, Ratchathewi, Bangkok, THAILAND.
Prapin Wilairat4Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, THAILAND., 5National Doping Control Center, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, THAILAND.
Chutima Matayatsuk Phechkrajang1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University; 447 Sri-Ayuthaya Road, Phayathai, Ratchathewi, Bangkok, THAILAND., 4Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, THAILAND., 6Center of Excellence for Innovation in Drug Design and Discovery, Faculty of Pharmacy, Mahidol University, Bangkok, THAILAND.
Objective: The mean centering of ratio spectra method (MCR) was developed for
determination of pirenoxine in the presence of methylparaben and propylparaben.
Background: The UV spectrum of pirenoxine was suffered from spectra overlapping of
methylparaben and propylparaben, the preservatives used in the eye drop formulation.
Since, MCR method was introduced to overcome this limitation. Methods: The developed
MCR method was performed using 39 synthetic mixtures of pirenoxine, methylparaben
and propylparaben. The amplitudes at 320 nm of the second ratio spectra were used to
construct a calibration model for pirenoxine. Performance characteristics of the method
such as linearity, accuracy and precision, were calculated. A high-performance liquid
chromatography (HPLC) method was also developed and validated. Then, two methods
were used to determine a set of commercial eye drop samples for comparison. Results:
The developed and validated MCR method was simple, rapid, accurate, and precise and
could be applied to determine pirenoxine in eye drop samples. Measurement of pirenoxine
in eye drop samples by MCR and HPLC methods were not significantly different (P-value
= 0.21).
