Evaluation of Clerodendrum inerme (L.) Gaertn. on Burkitt’s Lymphoma Cancer

Indian Journal of Pharmaceutical Education and Research

  • Chouhan Mahendra Kumar1Department of Pharmaceutical Biotechnology, College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, (Deemed-to-be University), Belagavi, Karnataka, INDIA., 2Dr. Prabhakar Kore Basic Science Research Center, KLE Academy of Higher Education and Research, (Deemed-to-be University), Belagavi, Karnataka, INDIA.
  • Hurkadale Pramod Jayadevappa1Department of Pharmaceutical Biotechnology, College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, (Deemed-to-be University), Belagavi, Karnataka, INDIA.
  • Hegde Harsha Vasudev3ICMR- National Institute of Traditional Medicine (Formerly Regional Medical Research Center), Belagavi, Karnataka, INDIA.

Volume 52 Issue 2 Pages 241-247

DOI: 10.5530/ijper.52.2.27

Abstract

According to current census every year six million deaths occur in the world due to cancer. Hence, there is need of discovery and development of novel, safe and efficacious drugs for the treatment of various cancer. In the present study, an attempt has been made to explore the medicinal aspects of Clerodendrum inerme (L.) Gaertn.. Verbenaceae plant involving cytotoxic and antiproliferative potential as hydroalcoholic extract using methanol and water (70:30 V/V). Preliminary phytochemical investigation confirms the presence of the moiety in hydro-alcoholic extract and cytotoxic analysis using brine shrimp lethality assay, Daudi cell line culture, dye exclusion assay, MTT assay and animal model. The extract showed cytotoxicity below 100 ppm and LD50 at 30 ppm level on brine shrimp lethality assay and on being subjected to MTT assay. A decrease in cell viability was observed at 213 μg/ml concentration and showing antitumor efficacy against Burkitt’s lymphoma cells as well tumor model in female mice which showing the increased life span, as compared to control group, when treated with C. inerme (200 and 400 mg/kg body weight) and doxorubicin (2.5 mg/kg body weight) exhibited significant effect on tumor parameters. Hence it can be stated that C. inerme has potent antitumor properties.

Keywords

  • Clerodendrum inerme
  • Cytotoxicity
  • Antiproliferative
  • Lethality
  • MTT
  • Daudi
IJOPP

Loading…