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IJPER · Lens · Development and Validation of UV-Spectrophotometric Method for Estimation of Metformin in Bulk and Tablet Dosage Form

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Article · Volume 51, Issue 4s (Supplement) · pp. s754-s760

Development and Validation of UV-Spectrophotometric Method for Estimation of Metformin in Bulk and Tablet Dosage Form

Yuvraj Dilip Dange1, Sandip Mohan Honmane1, Somnath Devidas Bhinge2, Vijay Rajaram Salunkhe2, Dhanraj Raghunath Jadge1

  1. 1Department of Pharmaceutics, Annasaheb Dange College of Pharmacy, Ashta, Shivaji University, Kolhapur, Maharashtra, INDIA.
  2. 2Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Shivaji University, Kolhapur, Maharashtra, INDIA.

Abstract

Introduction: Diabetes mellitus, a metabolic disorder characterized by increased blood sugar level. Metformin hydrochloride is used to treat type I Diabetes mellitus. Metformin hydrochloride chemically 1, 1-dimethylbiguanide hydrochloride, is white crystalline powder, hygroscopic and freely soluble in water, Officially UV spectrophotometric method used for estimation of Metformin Hydrochloride from the bulk and tablets formulations.Objective: Develop and validate a simple, rapid, accurate, economic and precise UV/VIS method for Metformin Hydrochloride in bulk and tablets formulation.Methodology: Choices of a common solvent were essential so various solvent ranges including methanol, ethanol, acetonitrile and phosphate buffer and various concentrations ranges of various buffers were analyzed.Conclusion: Among different solvents water has showed better results, hence water was selected as a solvent for the proposed method. Metformin Hydrochloride showed maximum absorbance at 234 nm. The percentage recoveries for Metformin Hydrochloride were found in the range of 99- 101 %. Method was quantitatively evaluated in terms of linearity, accuracy, precision, ruggedness, robustness and recovery. The method was simple, convenient and suitable for the determination of Metformin Hydrochloride from bulk and tablet dosage forms.

Keywords: Metformin HCl; UV-Spectrophotometry; Tablet

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