Comparative Evaluation of in vitro Cellular Uptake and Antiproliferative Potential of Different Extracts of Orthosiphon pallidus Royle

Indian Journal of Pharmaceutical Education and Research

  • Mukesh K. Singh1Shri Rawatpura Sarkar Institute of Pharmacy, Kumhari, Durg, Chhattisgarh, INDIA.
  • Hemant Dhongade1Shri Rawatpura Sarkar Institute of Pharmacy, Kumhari, Durg, Chhattisgarh, INDIA.
  • Dulal Krishna Tripathi2Rungta College of Pharmaceutical Sciences and Research, Kohka, Road, Bhilai, Durg, Chhattisgarh, INDIA.

Volume 51 Issue 4 Pages 722-728

DOI: 10.5530/ijper.51.4.106

Abstract

Objective: Evaluation of the cytotoxic effect of different extracts of Orthosiphon pallidus Royle on MCF-7 cell lines, comparatively. Materials and Methods: The breast cell line MCF-7 was cultured in DMEM medium containing fetal bovine serum & antibiotics. The cells were exposed to different doses of all the ethanolic, aqueous and hydroalcoholic extracts and incubated for 24, 48 and 72 hrs respectively and further studied for MTT colorimetric test, SRB test, glutathione assay and cellular uptake assay. Results: The results showed hydroalcoholic extract had a dose and time-dependent anticancer effect on MCF-7 cancer cells after 72 hours (P<0.05). The study revealed that Orthosiphon pallidus Royle could inhibit the growth of cancerous cells. Conclusion: The ethanolic, aqueous and hydroalcoholic extracts of Orthosiphon pallidus Royle exhibited anticancer activity in a wide range in dose -dependent manner. The study concluded that Orthosiphon pallidus Royle shows cellular uptake and anti-proliferative activities.

Keywords

  • Breast Cancer
  • Cytotoxic Effect
  • MCF-7 Cell Line
  • Fetal Bovine Serum
  • Glutathione’s
IJOPP

Loading…