The Influence of Methyl Jasmonate on Growth and Caffeic Acid Derivative Contents of In vitro Shoot and Roots in Echinaceae (Echinacea Purpurea)

Indian Journal of Pharmaceutical Education and Research

  • Tunhan Demirci1Süleyman Demirel University, Faculty of Agriculture, Department of Agricultural Biotechnology Isparta/TURKEY.
  • Serdar Özmen1Süleyman Demirel University, Faculty of Agriculture, Department of Agricultural Biotechnology Isparta/TURKEY.
  • Elif Gökcen Yılmaz1Süleyman Demirel University, Faculty of Agriculture, Department of Agricultural Biotechnology Isparta/TURKEY.
  • Özlem Aras Aşcı1Süleyman Demirel University, Faculty of Agriculture, Department of Agricultural Biotechnology Isparta/TURKEY.
  • Nilgün Göktürk Baydar1Süleyman Demirel University, Faculty of Agriculture, Department of Agricultural Biotechnology Isparta/TURKEY.

Volume 51 Issue 3s2 Pages s513-s517

DOI: 10.5530/ijper.51.3s.77

Abstract

This study was carried out to determine the effects of methyl jasmonate (MeJA) applications on growth and accumulation of caffeic acid derivatives (CADs) in shoots and roots grown in vitro. For this aim, 28 days old plants obtained from seeds were cultured in ½ Murashige and Skoog media containing different concentrations of MeJA (0, 10, 50 and 100 μM) and plants were harvested in three times at 15 days intervals. After harvest, growth parameters and CADs in shoot and roots were determined, separately. All growth parameters decreased in line with the elevating level of MeJA applications. But MeJA applications increased the CADs both shoots and roots compared to the controls. The highest cichoric acid, the main CAD of Echinacea, was found in shoots and roots applied with 100 μM MeJA and harvested in 45 days after application.

Keywords

  • Echinacea Purpurea
  • Methyl Jasmonate
  • Caffeic Acid Derives
  • Cichoric Acid
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