Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF

Indian Journal of Pharmaceutical Education and Research

  • Hai-bin Yu1The First Affiliated Hospital of Henan University of TCM, Zhengzhou 450000, China;.
  • LI Li2The Hospital of Henan Academy Institute of Traditional Chinese Medicine, Henan Zhengzhou 450000, China;.
  • Zhou-xin Ren3Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China;., 4Collaborative Innovation Center for Respiratory Disease Diagnosis And Treatment & Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.
  • Jun-ling Shen1The First Affiliated Hospital of Henan University of TCM, Zhengzhou 450000, China;.

Volume 50 Issue 4 Pages 583-590

DOI: 10.5530/ijper.50.4.10

Abstract

Transforming growth factor-β1 (TGF-β1) is a known potent inducer causing epithelial to mesenchymal transition(EMT) in some cancers. The present study was to evaluate the effect of hesperidin on EMT of non-small cell lung cancer (NSCLC) in vitro. In this study, A549 cells, a cellular line of non-small cell lung cancer, were used for evaluation of EMT induced by TGF-β1. MTT assay was used to evaluate the effect of hesperidin on cell viability with or without TGF-β1 co-culture. At the same time of TGF-β1 addition or after cellular morphological change by TGF-β1 stimulation, hesperidin was given to cells, respectively. Roundness of cells, E-cadherin, α-SMA, Col-I, MMP-9, TIMP-1 were evaluated EMT by TGF-β1 induction and effect of hesperidin on the transition. 40 and 80μM hesperidin showed significant inhibition on cellular viability without TGF-β1; co culture with TGF-β1, 40μM hesperidin showed significant inhibition on the viability but not showed significant inhibition after TGF-β1 stimulation. Whether co-culture with TGF-β1 or addition after TGF-β1, 40μM hesperidin showed inhibitory effect on epithelial to mesenchymal transition and inhibitory effect on extracellular matrix degradation of MMP-9. The above results suggest that hesperidin could be a potential candidate for inhibited migration of non-small cell lung cancer.

Keywords

  • Hesperidin
  • Non-small cell lung cancer
  • Transforming growth factor-β1
  • Proliferation
  • Epithelial to mesenchymal transition
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