Immunomodulatory Effect of Decalepis hamiltonii Wight and Arn Roots Extract on Rodents

Indian Journal of Pharmaceutical Education and Research

  • T. G. Sunitha1Sree Siddaganga College of Pharmacy, Tumkur-572102, INDIA.
  • Shivsharan Basavraj Dhadde1Sree Siddaganga College of Pharmacy, Tumkur-572102, INDIA., 3VT’s Shivajirao S. Jondhle College of Pharmacy, Asangaon-421 601, INDIA.
  • Sharanbasappa Durg1Sree Siddaganga College of Pharmacy, Tumkur-572102, INDIA.
  • Syed Mansoor Ahmed1Sree Siddaganga College of Pharmacy, Tumkur-572102, INDIA.
  • Boreddy Shivanandappa Thippeswamy1Sree Siddaganga College of Pharmacy, Tumkur-572102, INDIA., 4Department of Biomedical Science, College of Pharmacy, Shaqra University Al-Dawadmi, KINGDOM OF SAUDI ARABIA.
  • Veeresh Prabhakar Veerapur1Sree Siddaganga College of Pharmacy, Tumkur-572102, INDIA.
  • Baburao NCK2Department of Pharmaceutics, DSTS Mandal’s College of Pharmacy, Solapur-413 004, INDIA.

Volume 50 Issue 2s Pages s146-s152

DOI: 10.5530/ijper.50.2.30

Abstract

Background: Decalepis hamiltonii Wight and Arn (Asclepiadaceae) commonly known as ‘nannari’ is an Indian medicinal plant which is having ethno-medicinal uses as antioxidant, tonic and blood purifier. It is used in many Ayurvedic preparations like Drakshadi churna (general vitalizer), Shatavari rasayana (adaptogenic), and Yeshtimadhu taila (mild analgesic, rheumatism). Aim of the present study is to evaluate the immunomodulatory activity of D. hamiltoni roots aqueous extract in rodents. Methods: D. hamiltoni aqueous extract (DHAE) was prepared by cold maceration. Two doses of DHAE (100 and 200 mg/kg, per os) were used to evaluate immunomodulatory activity in vivo and ex vivo through assessments of hemagglutination antibody titers, hematological profiles (Hb, WBC, RBC), delayed-type hypersensitivity (DTH), neutrophil adhesion, and carbon clearance. Results: DHAE at both dose levels evoked significant dose-dependent increases in antibody titers and DTH reactions induced by SRBC, and potentiated percentage neutrophil adhesion to nylon fibers as well as phagocytosis of carbon particles in situ. Conclusion: The evidence collected here lends support to traditional claims behind the use of D. hamiltonii for medicinal purposes. The study also affirmed that DHAE imparts immunomodulatory activity. The DHAE not only potentiated non-specific immune responses, but also was effective in improving immune responses in cyclophosphamide- immunosuppressed hosts. The results here indicate that D. hamiltonii root extracts stimulates both cellular and humoral immune responses.

Keywords

  • Nannari
  • Cyclophosphamide
  • Hemagglutination antibody titer
  • Neutrophil adhesion test
  • Carbon
  • clearance assay
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