Simultaneous Estimation of Anions Present in Oral Rehydration Salt by ION Chromatography and its Method Validation

Indian Journal of Pharmaceutical Education and Research

  • Preeti Kulkarni1C. U. Shah College Of Pharmacy, S. N. D. T. Women's University, Santacruz (W), Mumbai-400 049, India.
  • Sonal Desai1C. U. Shah College Of Pharmacy, S. N. D. T. Women's University, Santacruz (W), Mumbai-400 049, India.
  • Pratima Tatke1C. U. Shah College Of Pharmacy, S. N. D. T. Women's University, Santacruz (W), Mumbai-400 049, India.
  • T. P. Ghule2Analytical Solutions, R-610, Rabale, M. I. D. C., Mumbai-400 701, India.
  • C.D. Chavan2Analytical Solutions, R-610, Rabale, M. I. D. C., Mumbai-400 701, India.
  • V. R. Bhate2Analytical Solutions, R-610, Rabale, M. I. D. C., Mumbai-400 701, India.

Volume 47 Issue 2 Pages 183-187

Abstract

The present study aims at development of method for simultaneous estimation of chloride and citrate in oral rehydration salt (ORS) by ion chromatography and its validation. The estimation was carried out with a step change gradient mode by altering concentrations of NaOH as an eluent, an anion exchange column IonPac AS11 analytical column, guard column AG11 and conductivity detector. The suppressor used was used as ASRS ULTRA. The developed method was validated as per ICH guidelines for various parameters. The method is useful for determining the contents of chloride and citrate in single run from ORS.

Keywords

  • Ion chromatography
  • Oral rehydration salt
  • simultaneous estimation
  • chloride
  • citrate
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