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Published on:April 2019
Indian Journal of Pharmaceutical Education and Research, 2019; 53(2s):s170-s178
Original Article | doi:10.5530/ijper.53.2s.63

Simultaneous Determination of Hydrophilic and Lipophilic Drugs in Anti-Cancer Liposomes: Absorptivity Method

Authors and affiliation (s):

Harshita Mishra1, Pawan Kumar Mishra2, Manu Jaggi3, Zeenat Iqbal1, Sushama Talegaonkar1,4*

1School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, INDIA.

2Department of Wood Processing Technology, Mendel University in Brno, Brno, CZECH REPUBLIC.

3Dabur Research Foundation, Ghaziabad, Uttar Pradesh, INDIA.

4Department of Pharmaceutics, Delhi Pharmaceutical Sciences and Research University, Govt. of NCT of Delhi, New Delhi, INDIA.


Background: Dual loaded liposomes have always been a hot topic of investigation because of their therapeutic advantages over single drug regimens. But simultaneous estimation of both the drugs during in vitro and in vivo analysis is most of the times a major obstacle for the researchers. This problem is even bigger when the two drugs are of opposite nature (hydrophilic and lipophilic). Methods: Here, we formulated anti-cancer liposomes loaded with Dacarbazine (hydrophilic) and Eugenol (lipophilic). Since both the drugs are of different nature, selecting a solvent system that dissolves both the drugs, as well as is a suitable media for determining in vitro release, was a challenge. After resolving this issue, we developed a simple and robust UV method based on the principle of solving simultaneous equations generated after Absorptivity measurements of the two drugs. Results: UV spectrophotometer is preferred over other analytical techniques because of its availability, broad area of applications, easy and fast handling, robust nature and simplicity. Thus, using the developed method for simultaneous determination, loading of the two drugs and their simultaneous release from the liposomes was determined. Conclusion: We could perform the in-vitro characterization of formulated anti-cancer liposomes using the developed UV Absorptivity method, which justifies the practical applicability of the developed method and proved that the method is specific and reproducible.

Key words: UV absorptivity method, Simultaneous determination, Dual loaded liposomes, Suitable solvent system, Method Validation.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
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Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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