16S rDNA sequencing of Antibiotic Producing Bacterial Strain Lysinibacillus sp.(BP-3) from Nagavali River Basin of Srikakulam.

Abstract:

Antibiotic producing bacteria Lysinibacillus sp.(BP-3) was isolated from the Nagavali river basin, Srikakulam, Andhra pradesh, India. The whole cell protein extract of BP-3 strain was found to have significant inhibitory effect on the test strains viz: E.coli (MTCC 40), Staphlococcus aureus (MTCC87),Proteus vulgaris(MTCC426), and Pseudomonas aeruginosa (MTCC 424). Identification of the bacteria was carried out using biochemical tests, 16SrRNA sequencing and sequences submitted to the NCBI GenBank Maryland USA and awarded with the accession number HM359123. Strain BP-3 exhibited maximum inhibitory effect and was identified as Lysinibacillus sp. Nagavali river basin receives a very high number of microbes from the dense virgin forests of Orissa and Andhra Pradesh during the first monsoon therefore it is of a great importance to screen the microbes for the hunt of a novel antibiotic producing strain from the site and characterize it at molecular level to evaluate its potential as candidate strain for antibiotic production.

Keywords: Nagavali, antibiotic, 16SrRNA sequencing, Lysinibacillus