Amelioration of Metabolic Syndrome in High Fat Fed Mice by Hydro-Ethanolic Fraction of M. longifolia (J. Koenig)

Abstract:

Objective: To investigate the effect of hydro-ethanolic fraction of leaves of M. longifolia (J. Koenig) on high fat diet induced metabolic disorder. Methods: The M. longifolia extract was obtained from the successive extraction followed by fractionation. Ex vivo glucose uptake study was performed. Body weight and blood glucose was checked at regular intervals. After euthanasia serum biochemical parameters, lipid parameters were estimated. Organs were isolated, weighed and fixed in formalin for histopathological analysis. Results: Compared to control, M. longifolia leaf fractions showed significant glucose uptake inhibition in ex vivo studies (rat hemi diaphragm and rat intestinal glucose uptake assay). HFD group showed significant weight increase as compared to normal control. Blood glucose level and glycosylated haemoglobin was found elevated in disease group (obesity and diabetic group). Significant increase in lipid profile of disease group aggravates the diabetic condition. Animal treated with M. longifolia leaf fractions (400mg/kg) helps in normalisation of liver and white adipose tissue. Conclusion: Metabolic syndrome is an agglomeration of disorders inclusive of increased blood glucose, excess body fat, anomalous triglyceride and cholesterol level, which on joint occurrence leads to diabetes and increased risk of heart disease. Our data suggest that treatment with M. longifolia to HFD + STZ group alleviates the diabetes and helps in control of obesity. It may be due to presence of various bioactive constituents.

Key words: M. longifolia, Ex vivo, Metabolic disorder, In vivo, Blood glucose, BMI.