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Published on:August 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(4s):s86-s90
Original Article | doi:10.5530/ijper.52.4s.80

Seasonal Changes of Phagocytic Activity Enhanced by an Acoholic Mint Extract in Cultured Rainbow Trout

Authors and affiliation (s):

Spînu Marina, Pall Emoke, Niculae Mihaela, Vasiu Aurel , Brudaşcă Gheorghe Florinel , Cerbu Constantin Gheorghe, Negrutiu Vlad, Vasiu Constantin, Silvana Popescu, Sandru Carmen Dana

Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.


Objective: We hypothesized that leukocyte numbers and their in vitro phagocytic activity in farmed rainbow trout are subject to negative influence of temperatures extreme for fish and that mint extract could alleviate these effects. Materials and Methods: Blood samples from farmed rainbow trout collected during winter and summer were subjected to leukocyte subpopulation counts (%) and carbon particle inclusion test to estimate the in vitro phagocytic activity after 15 and 30 min of incubation at 4 and 22°C, with or without alcoholic mint extract (ln). Results and Discussion: There was a significant increase in lymphocytes (p<0.05) and monocytes (p<0.01) in summer versus winter, while the heterophils were dominant during winter (p<0.01). In vitro spontaneous phagocytosis increased with the seasonal increase in temperature (0.425±0.094, winter and 0.835±0.102, summer, p<0.05) and during the first reading period (0 to 15 min, p<0.01). The mint extract increased phagocytosis for the overall reading period from 2x (winter, 0.281±0.221) up to 5x (summer, 1.032±0.221) (p<0.01). Conclusion: The positive activity of the alcoholic mint extract during phagocytosis, more intense during summer than during spring, proved to be a temperature driven process, plant active principles playing a dual stimulating role for both monocytes and heterophiles.

Key words: Rainbow trout, Season, Leukocytes, Phagocytosis, Mint extract.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
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Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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