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Published on:January 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(2):321-326
Original Article | doi:10.5530/ijper.52.2.37

Estimation of Phenolic Compounds Present in the Plant Extracts Using High Pressure Liquid Chromatography, Antioxidant Properties and its Antibacterial Activity

Authors and affiliation (s):

Panchaksharam Supritha, Kuravappullam Vedaiyan Radha*

Department of Chemical Engineering, Bio-products Laboratory, AC Tech, Anna University, Chennai, Tamil Nadu, INDIA.


Objective: The aim of the present study was to evaluate total phenolic content of the prepared plant extracts and investigation of phenolic compounds present in the extract. Background: The method was done to evaluate the phenolic compounds and finding the components presence. Methods: The extraction was carried out using methanol as the solvent. Plants used in this analysis were Acalypha indica, Azadirachta indica, Lawsonia inermis and Murraya konegii. Qualitative estimation of total phenol content was done using Folin- Ciocalteu method in with gallic acid was used as standard. HPLC analysis was done to reveal the nature of phenolic compounds, C18 column was used. Antibacterial activity was carried out using agar diffusion method. Antioxidant studies were done using DPPH assay. Results: Antibacterial assay revels Lawsonia inermis showed highest zone of inhibition on Proteus sps. The high amount of phenolic content was obtained in Lawsonia inermis (5.2 μg/ml) and the lowest was Murraya konegii (2.3 μg/ml). HPLC of various extracts revel the presence of simple phenols, catechins, anthocyanins, aglycons, anthraquinones, glycosides form of flavones, flavonols, isoflavones and flavanones. DPPH assay revels that Lawsonia inermis has good antioxidant properties compared to other extracts.

Key words: HPLC, Folin- Ciocalteu, Extraction, Simple phenols, Catechins.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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