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Published on:September 2017
Indian Journal of Pharmaceutical Education and Research, 2017; 51(3s2):s498-s503
Original Article | doi:10.5530/ijper.51.3s.74

In vitro Anticancer Activity and Antioxidant Properties of Essential Oils from Populus alba L. and Rosmarinus officinalis L. from South Eastern Anatolia of Turkey

Authors and affiliation (s):

Sevgi Gezici1, Nazim Sekeroglu2, Anake Kijjoa3

1Department of Biology, University of Gaziantep, Gaziantep, Turkey, sevgigezici.00@gmail.com

2Department of Medicinal and Aromatic Plants, University of Kilis 7 Aralik, Kilis, Turkey, nsekeroglu@gmail.com

3Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Porto, Portugal, ankijjoa@icbas.up.pt


Background and Purpose: In recent years, essential oils (EOs) have been reported to possess interesting anti-tumor, anti-mutagenic and anti-carcinogenic activities against various cancer cells. Therefore, we aimed to investigate potential biological activities of EOs from white poplar (Populus alba L., Salicaceae) and rosemary (Rosmarinus officinalis L., Lamiaceae). Material and Methods: EOs from P. alba L. and R. officinalis L. were extracted by hydrodistillation. MTT assay was carried out to determine the potential antiproliferative and cytotoxic properties of the essential oils as well as their corresponding IC50, and the inhibition (%) calculated. Antioxidant activity was determined using 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay, and lipid peroxidation capacity was evaluated using thiobarbituric acid-reactive substances (TBARS) method, and the values were calculated using the standards. Results: The EOs were evaluated for their in vitro cytotoxic, antioxidant and lipid oxidation activities. Regarding cytotoxic activity rosemary essential oil possessed strong inhibition (IC50 = 3.06-7.38 μg/mL) of cell proliferation in comparison to that of P. alba L. (IC50 = 10.53-28.16 μg/mL). Additionally, EO from R. officinalis L. was found to have higher antioxidant and lipid peroxidation capacities with IC50 of 10.08 ± 0.15 and 1.76 ± 0.01, respectively. Conclusion: The results suggest that the EOs of both sources exhibited strong antiproliferative, cytotoxic and potent antioxidant properties and therefore they can have potential applications in the cancer treatment.

KEY WORDS: Cytotoxicity, Populus alba L., Rosmarinus officinalis L., DPPH, EOs, MTT, TBARS, IC50.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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