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Published on:July 2017
Indian Journal of Pharmaceutical Education and Research, 2017; 51(3s):s170-s175
Original Article | doi:10.5530/ijper.51.3s.6

The Antibacterial Activities of Piper nigrum L. Against Mastitis Pathogens and its Antioxidant Activities

Authors and affiliation (s):

Gulten Okmen*, Mustafa Vurkun, Ali Arslan, Olcay Ceylan

Faculty of Science, Department of Biology, Mugla Sitki Kocman University, Kotekli Mugla 48000, TURKEY


Objective / Purpose: Bacteria causes one of the most common types of chronic mastitis. The most common causative organisms of mastitis include: Staphylococci, Streptococci and coliforms. The scope of this work was to research the antibacterial effects of Piper nigrum extracts against mastitis pathogens, and its antioxidant capacity. Materials and Methods: In our study, 2 Staphylococcus aureus and 5 Coagulase Negative Staphylococcus were used for experiments. Additionally, Piper nigrum were collected from Mugla herbalists in Turkey. The plant extracts were tested by disc diffusion assay for antibacterial activity. The antioxidant activities of plant extracts were also determined by ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] method. Results: The methanol extract of plant showed maximum inhibition zone against 2 bacteria. MIC values of extracts are 6500 μg/mL. The value found in ABTS method is highly effective (75.7%). Discussion/Conclusion: As a result, the in vitro studies indicate that the extracts of Piper nigrum have significant antibacterial and antioxidant activities. In addition to, the plant extracts could be used in treating mastitis caused by the test bacteria.

Key words: Piper, Mastitis, Staphylococcus, Antibacterial Activity, Antioxidant Activity.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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