Insilico Design, Synthesis of Hybrid Taurine Amino Acid and Peptide Analogues for Studies on Antioxidant and Hepatoprotective Activity

Abstract:

Introduction: The liver performs normal metabolic homeostasis of the body as well as biotransformation, extraction and detoxification of many compounds. Due to this it is more susceptible to disease. Near about 900 drug are withdraw from the market due to hepaptoxicity. The objective of the work to synthesis the series of hybrid taurine amino acid and peptide analogues in which the various combinations of taurine amino acid, Di-peptide and Tri- peptide were synthesized. Aims: In this study, we mainly focused on the hepatoprotective aspects of hybrid taurine amino acid peptides analogues before the synthesis we carry out the insilico designing of molecules and from the results of this insilico study we carry forward the synthesis of hybrid compound followed by their in vitro and in vivo studies. Results: the binding affinity of the designed compound towards CYP2E1 (3GPH) was selected on the basis docking score The compound SSSB-16 shows the maximum score having the docking score is -24.84 as compared with single taurine and other taurine hybrid compound. The compound SSSB 15 is second in the list of docking score with the docking score is -24.67. The reference ligand .having the docking score is -11.90. All the compounds were screened for their in vitro antioxidant activity by employing DPPH, Nitric oxide scavenging method. From the in vitro result of antioxidant activity those compound which had shown maximum activity till use for hepatoprotective activity. The compound SSSB 3 which is the combination of Taurine- Glycine-Glycine shows the maximum activity as compared to all other compounds. Conclusion: From result good activity was noted for SSSB3 (Taurine-Gly-Gly) compound. From this it can be concluded that the amino acid hybrid with future being proof to be novel compound as hepatoprotective activity. It may be use as a supplement with the drugs to reduced hepatotoxicity.

Key words: Taurine, Peptides, Cyp2e1, Antioxidant, Hepatoprotection.